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An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

3.0K Views

09:52 min

September 15th, 2020

DOI :

10.3791/61648-v

September 15th, 2020


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In Vitro

Rozdziały w tym wideo

0:05

Introduction

0:51

Equipment Preparation

3:11

3’-End Biotinylated mRNA Immobilization

5:20

Translation Mix Assembly and Delivery

7:24

Results: Analyses for Saccharomyces cerevisiae Translation of a Firefly Luciferase-encoding mRNA

9:12

Conclusion

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