JoVE Logo

Zaloguj się

Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

6.6K Views

07:02 min

November 3rd, 2020

DOI :

10.3791/61605-v

November 3rd, 2020


Przeglądaj więcej filmów

Actinin Network

Rozdziały w tym wideo

0:04

Introduction

0:27

Photoactivated Localization Microscopy (PALM) Image Acquisition Setup

1:27

PALM Image Acquisition

1:50

PALM Data Reconstruction

2:39

Reconstructed PALM Image Post Processing

3:34

Sarcomere Filament Analysis

4:41

Results: Representative Human Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte alpha-Actinin Network Analysis

6:23

Conclusion

Powiązane Filmy

article

10:56

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

21.1K Views

article

09:41

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

8.6K Views

article

10:30

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

20.5K Views

article

10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

6.3K Views

article

08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

4.0K Views

article

08:03

Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

1.7K Views

article

13:18

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

21.2K Views

article

05:06

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

12.9K Views

article

08:37

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

4.3K Views

article

06:59

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

7.1K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone