A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
Please note that all translations are automatically generated. Click here for the English version.
5.4K Views
•
09:50 min
•
June 17th, 2020
DOI :
June 17th, 2020
•Hussein Atta*1,2, Ali Hashemi Gheinani*1,2, Amanda Wacker1, Yaser Heshmati3,4,5, Alex Bigger-Allen1,6, George Lambrinos1,2, Yao Gao2,7, Diane R. Bielenberg2,7, Rosalyn M. Adam1,2
1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital
Rozdziały w tym wideo
0:04
Introduction
0:57
Mouse Preparation
1:48
Spinal Cord Injury (SCI)
3:17
Tissue Perfusion and Procurement
4:34
Cell Dissociation and Single Cell Suspension Preparation
5:59
Cell Staining
6:44
Flow Cytometry Analysis
7:43
Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
8:54
Conclusion
Powiązane Filmy
03:48
Mouse Bladder Wall Injection
23.9K Views
09:16
Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells
2.6K Views
10:23
Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice
16.4K Views
08:10
Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
47.7K Views
07:34
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
8.0K Views
09:08
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
9.8K Views
06:18
Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
3.8K Views
07:14
Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
3.6K Views
08:49
Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice
1.5K Views
06:45
Isolation of Pure Astrocytes and Microglia from the Adult Mouse Spinal Cord For In Vitro Assays and Transcriptomic Studies
1.6K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone