Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
Please note that all translations are automatically generated. Click here for the English version.
17.0K Views
•
07:19 min
•
February 18th, 2020
DOI :
February 18th, 2020
•Rozdziały w tym wideo
0:05
Introduction
0:50
Organoid Culture and Preparation
2:23
Electroporation
3:33
Cell Seeding
4:05
Determination of Transfection Efficiency
5:18
Results: Electroporation Efficiency of Four Organoid Entities
6:47
Conclusion
Powiązane Filmy
07:37
Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
8.1K Views
11:53
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
18.0K Views
10:49
Laboratory-Engineered Glioblastoma Organoid Culture and Drug Screening
453 Views
11:44
Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification
3.3K Views
07:13
Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
926 Views
09:18
A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
29.6K Views
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
30.5K Views
10:49
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
2.6K Views
09:49
Generation and Manipulation of Rat Intestinal Organoids
3.8K Views
02:16
Nokaut wielu genów za pośrednictwem CRISPR: technika jednoczesnego nokautowania wielu genów za pomocą niehomologicznego szlaku łączenia końców w komórkach jelitowych myszy
1.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone