JoVE Logo

Zaloguj się

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

6.7K Views

10:43 min

November 6th, 2019

DOI :

10.3791/60398-v

November 6th, 2019


Przeglądaj więcej filmów

Oligomerization Dynamics

Rozdziały w tym wideo

0:04

Title

1:48

Sample Preparation

2:38

TIRF Imaging: Alignment of the Laser Line and Optimization of TIRF Illumination

4:05

TIRF Imaging: Capture of the Time Series

5:46

Number & Brightness (N&B): Quality Check of the Time Series

6:34

Number & Brightness (N&B): Computation of the B-values in Selected Region-of-interest (ROI)

8:19

Results: N&B Analysis and Kinetics of FGFR1 Oligomerization

9:42

Conclusion

Powiązane Filmy

article

14:14

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

11.4K Views

article

08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

7.2K Views

article

08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

11.2K Views

article

15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

11.3K Views

article

09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

7.9K Views

article

15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

11.3K Views

article

20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

13.8K Views

article

10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

12.8K Views

article

10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

3.1K Views

article

12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

8.6K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone