Zaloguj się

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

7.0K Views

08:29 min

November 1st, 2019

DOI :

10.3791/60098-v

November 1st, 2019


Przeglądaj więcej filmów

SUMO binding Entities

Rozdziały w tym wideo

0:04

Title

1:58

Cell Preparation and Lysis

3:27

Tissue Preparation and Lysis

3:54

Binding of GST-SUBEs or GST Control to Glutathione-Agarose Beads

4:52

GST Pull Down Assay

5:59

Identification and Characterization of SUMO Targets

6:36

Results: Western Blot and Mass Spectrometry Analysis

7:33

Conclusion

Powiązane Filmy

article

10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

11.8K Views

article

12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

12.0K Views

article

06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

8.4K Views

article

08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

16.0K Views

article

10:12

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

2.6K Views

article

09:22

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

24.8K Views

article

12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

31.4K Views

article

11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

2.4K Views

article

09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

7.2K Views

article

09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

9.3K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone