Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
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09:14 min
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May 7th, 2019
DOI :
May 7th, 2019
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Rozdziały w tym wideo
0:04
Title
1:00
Sectioning of Human Skin Dermis into Papillary and Reticular Layers
2:38
Separation and Enzymatic Digestion of Epidermis and Dermis
3:52
Preparation of Single Cell Suspension and Erythrocyte Lysis
5:58
Culturing of Fibroblasts and Adipogenesis Assay
7:04
Results: FAP+CD90-, FAP+CD90+ and FAP-CD90+ Fibroblasts Differ in Dermal Localization, Gene Expression and Functionality
8:44
Conclusion
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