Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
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14.6K Views
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11:48 min
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April 24th, 2018
DOI :
April 24th, 2018
•Yannig Gicquel*1,2, Robin Schubert*3,4,5, Svetlana Kapis3, Gleb Bourenkov6, Thomas Schneider6, Markus Perbandt3,4, Christian Betzel3,4,5, Henry N. Chapman1,2,4, Michael Heymann1,7
1Center for Free Electron Laser Science, DESY, 2Department of Physics, University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, 4The Hamburg Center for Ultrafast Imaging, University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
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Title
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Polydimethylsiloxane (PDMS) Mold Fabrication and In Situ X-ray Chip Fabrication
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Fluid Delivery Access Ports and Surface Treatment
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Protein Preparation and Crystallization
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Results: Crystallization and In Situ X-ray Diffraction of Protein Crystals
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Conclusion
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