An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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10.3K Views
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10:41 min
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April 5th, 2018
DOI :
April 5th, 2018
•Rozdziały w tym wideo
0:04
Title
0:31
Tissue/Cell Line Processing and Cross-linking
1:57
Tissue Lysis, Sonication, and Antibody Preparation
2:52
Chromatin Immunoprecipitation
3:40
Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
4:43
Purification and Quantification of Precipitated DNA
6:00
Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
7:53
Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
9:23
Conclusion
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