JoVE Logo

Zaloguj się

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

6.6K Views

11:53 min

June 1st, 2018

DOI :

10.3791/56391-v

June 1st, 2018


Przeglądaj więcej filmów

Mouse Embryonic Stem Cells

Rozdziały w tym wideo

0:04

Title

0:57

Grow Mouse ES Cells on Gelatin-coated Dishes

2:48

Extract DNA from Cells and Enzymatic Digestion of DNA into Single Nucleosides

5:29

Analyze 5mC and 5hmC Using UHPLC-MS/MS

8:34

Results: Methylation Status of Mouse Embryonic Stem Cells

10:17

Conclusion

Powiązane Filmy

article

11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

7.1K Views

article

09:32

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

10.2K Views

article

08:27

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

6.3K Views

article

15:13

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

10.9K Views

article

09:04

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

8.9K Views

article

08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

6.5K Views

article

11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

9.7K Views

article

10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

6.3K Views

article

11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

21.4K Views

article

08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

12.3K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone