High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
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14:21 min
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October 19th, 2017
DOI :
October 19th, 2017
•Rozdziały w tym wideo
0:05
Title
1:09
PCR Amplification of cDNA Templates from Plasmids
3:43
In vitro Transcription to Generate Antisense Probes
5:25
Drosophila Tissue Collection and Fixation
7:51
In Situ Hybridization
9:05
Probe Detection
11:05
Antibody Detection using Tyramide
12:01
Results: Robust Detection of RNA in Whole Drosophila Embryos and Dissected Tissues
13:02
Conclusion
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