JoVE Logo

Zaloguj się

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

9.6K Views

11:01 min

November 23rd, 2016

DOI :

10.3791/54652-v

November 23rd, 2016


Przeglądaj więcej filmów

Activity based Probe

Rozdziały w tym wideo

0:05

Title

1:11

Preparation of 2-pyridyl Undec-10-ynyl Carbonate and Undec-10-ynyl 2-oxopyridine 1-carboxylate

4:17

Click Chemistry with Azide-PEG3-Biotin

5:14

Removal of Excess Click Chemistry Reagents

6:29

Streptavidin Enrichment

7:57

Click Chemistry with Azide-PEG3-Fluor 545 on Tissue Slices

8:59

Results: Synthesis and Applications of NAAA Activity Based Probe, ARN14686

10:21

Conclusion

2:44

Preparation of Undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate

Powiązane Filmy

article

11:16

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

16.1K Views

article

09:07

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

6.7K Views

article

16:33

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

35.7K Views

article

07:37

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

1.9K Views

article

10:24

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

9.9K Views

article

05:51

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase

23.0K Views

article

11:06

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

16.9K Views

article

10:16

In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease

8.0K Views

article

09:33

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

9.8K Views

article

08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

8.7K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone