A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
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11:01 min
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November 20th, 2014
DOI :
November 20th, 2014
•Samantha L. Eaton1, Maica Llavero Hurtado1, Karla J. Oldknow2, Laura C. Graham1, Thomas W. Marchant1, Thomas H. Gillingwater3,4, Colin Farquharson2, Thomas M. Wishart1,4
1Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 2Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 3Centre for Integrative Physiology, University of Edinburgh, 4Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh
Rozdziały w tym wideo
0:05
Title
1:32
Sample Preparation and Loading
3:43
iBlot2 Semi Dry Fast Protein Transfer
4:54
Post Transfer Membrane Handling
6:32
Visualization
7:51
Quantification of Scanned Proteins
8:50
Results: Representative Quantitative Fluorescent Western Blotting Experiments
10:31
Conclusion
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