Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
Please note that all translations are automatically generated. Click here for the English version.
79.1K Views
•
09:59 min
•
February 12th, 2014
DOI :
February 12th, 2014
•Sarah H. Shahmoradian1, Mauricio R. Galiano2, Chengbiao Wu3, Shurui Chen4, Matthew N. Rasband2, William C. Mobley3, Wah Chiu4
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Neuroscience, Baylor College of Medicine, 3Department of Neuroscience, University of California at San Diego, 4National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Rozdziały w tym wideo
0:05
Title
1:19
Preparing Dishes with EM Grids
3:43
Preparing and Plating Primary Neurons on EM Grids
4:45
Vitrifying Neurons on EM Grids
8:05
Results: Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
9:32
Conclusion
Powiązane Filmy
04:35
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
16.2K Views
15:00
Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
5.6K Views
07:00
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
3.2K Views
09:06
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
4.1K Views
09:30
Super-resolution Imaging of Neuronal Dense-core Vesicles
9.7K Views
09:53
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
6.4K Views
12:27
Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
6.9K Views
11:55
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
14.0K Views
08:47
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
3.7K Views
08:37
Visualization of Organelles In Situ by Cryo-STEM Tomography
1.9K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone