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Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

11.6K Views

13:47 min

December 10th, 2013

DOI :

10.3791/50625-v

December 10th, 2013


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Keywords In Vitro Model

Rozdziały w tym wideo

0:00

Title

1:17

Preparation of THP-1 Macrophage Monolayer

2:59

Preparation of Candida glabrata Cell Suspension

4:26

Infection of THP-1 Macrophages with C. glabrata Cells

5:52

Measurement of Phagocytosis Rate and Intracellular Replication via Colony Forming Unit Assay

7:20

Monitoring of Intracellular Replication Using Confocal Laser Scanning Microscopy

9:07

Representative Results

9:33

Conclusion

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