JoVE Logo

Zaloguj się

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

30.5K Views

10:22 min

August 15th, 2013

DOI :

10.3791/50541-v

August 15th, 2013


Transkrypcja

Przeglądaj więcej filmów

Protein Purification

Rozdziały w tym wideo

0:05

Title

1:43

Preparation of Cell Lysate

4:02

Microscale Thermophoresis Binding Studies

7:05

Microscale Thermophoresis Data Analysis

8:25

Results: Measuring the Affinity of Non-phosphorylated STAT3 Protein Binding to Oligonucleotides

9:40

Conclusion

3:20

Determination of Optimal Lysate Dilution

Powiązane Filmy

article

08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

10.5K Views

article

14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

17.8K Views

article

10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

12.0K Views

article

04:45

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

6.7K Views

article

10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

4.2K Views

article

10:04

Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms

12.7K Views

article

11:21

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

624 Views

article

10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

37.5K Views

article

13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

61.5K Views

article

04:30

Tandemowy test oczyszczania powinowactwa do badania interakcji białko-białko

63 Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone