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Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay

31.7K Views

12:52 min

March 3rd, 2011

DOI :

10.3791/2631-v

March 3rd, 2011


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Detection

Rozdziały w tym wideo

0:00

Title

2:56

Plating of Cells and Treatment with BMP

4:12

Fixation and Permeabilization

10:56

In Situ Proximity Ligation Assay Results from Neuro2a Cells Treated with BMP4

6:49

The Proximity Ligation Assay (PLA)

5:26

Blocking and Primary Antibodies Application

9:54

Preparation for Imaging

12:18

Conclusion

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