Name: biodegradable magnesium stent treatment of saccular aneurysms in a rat model - introduction of the surgical technique
Uploaded: 2017-10-01T13:00:00.000+00:00
Duration: 11 min 7 s
Description: Watch this Scientific Journal Video about Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique at JoVE.com

우리 감사 합니다 오이겐 호프만 Philine Zumstein에 그들의 우수한 기술 지원을 텐트 신청 절차에서 자신의 전문성을 공유. 우리 해 부 드로잉 Majlinda Kalanderi 감사합니다.

<ol>
	<li>Molyneux, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+Subarachnoid+Aneurysm+Trial+(ISAT)+of+neurosurgical+clipping+versus+endovascular+coiling+in+2143+patients+with+ruptured+intracranial+aneurysms:+a+randomised+trial.">International Subarachnoid Aneurysm Trial (ISAT) of neurosurgical clipping versus endovascular coiling in 2143 patients with ruptured intracranial aneurysms: a randomised trial.</a> Lancet. 360 (9342), 1267-1274 (2002).</li><li>Molyneux, A. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+subarachnoid+aneurysm+trial+(ISAT)+of+neurosurgical+clipping+versus+endovascular+coiling+in+2143+patients+with+ruptured+intracranial+aneurysms:+a+randomised+comparison+of+effects+on+survival,+dependency,+seizures,+rebleeding,+subgroups,+and+aneurysm+occlusion.">International subarachnoid aneurysm trial (ISAT) of neurosurgical clipping versus endovascular coiling in 2143 patients with ruptured intracranial aneurysms: a randomised comparison of effects on survival, dependency, seizures, rebleeding, subgroups, and aneurysm occlusion.</a> Lancet. 366 (9488), 809-817 (2005).</li><li>Marbacher, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraluminal+cell+transplantation+prevents+growth+and+rupture+in+a+model+of+rupture-prone+saccular+aneurysms.">Intraluminal cell transplantation prevents growth and rupture in a model of rupture-prone saccular aneurysms.</a> Stroke. 45 (12), 3684-3690 (2014).</li><li>Marbacher, S., Marjamaa, J., Abdelhameed, E., Hernesniemi, J., Niemela, M., Frosen, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Helsinki+rat+microsurgical+sidewall+aneurysm+model.">The Helsinki rat microsurgical sidewall aneurysm model.</a> J Vis Exp. (92), e51071 (2014).</li><li>Marbacher, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Loss+of+mural+cells+leads+to+wall+degeneration,+aneurysm+growth,+and+eventual+rupture+in+a+rat+aneurysm+model.">Loss of mural cells leads to wall degeneration, aneurysm growth, and eventual rupture in a rat aneurysm model.</a> Stroke. 45 (1), 248-254 (2014).</li><li>Pritchett-Corning, K. R., Luo, Y., Mulder, G. B., White, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+rodent+surgery+for+the+new+surgeon.">Principles of rodent surgery for the new surgeon.</a> J Vis Exp. (47), (2011).</li><li>Bernal, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Guidelines+for+rodent+survival+surgery.">Guidelines for rodent survival surgery.</a> J Invest Surg. 22 (6), 445-451 (2009).</li><li>Flege, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+and+characterization+of+a+coronary+polylactic+acid+stent+prototype+generated+by+selective+laser+melting.">Development and characterization of a coronary polylactic acid stent prototype generated by selective laser melting.</a> J Mater Sci Mater Med. 24 (1), 241-255 (2013).</li><li>Zartner, P., Cesnjevar, R., Singer, H., Weyand, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=First+successful+implantation+of+a+biodegradable+metal+stent+into+the+left+pulmonary+artery+of+a+preterm+baby.">First successful implantation of a biodegradable metal stent into the left pulmonary artery of a preterm baby.</a> Catheter Cardiovasc Interv. 66 (4), 590-594 (2005).</li><li>Hakamata, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Green+fluorescent+protein-transgenic+rat:+a+tool+for+organ+transplantation+research.">Green fluorescent protein-transgenic rat: a tool for organ transplantation research.</a> Biochem Biophys Res Commun. 286 (4), 779-785 (2001).</li><li>Bouzeghrane, F., Naggara, O., Kallmes, D. F., Berenstein, A., Raymond, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+Consortium+of+Neuroendovascular+C.+In+vivo+experimental+intracranial+aneurysm+models:+a+systematic+review.">International Consortium of Neuroendovascular C. In vivo experimental intracranial aneurysm models: a systematic review.</a> AJNR Am J Neuroradiol. 31 (3), 418-423 (2010).</li><li>Aquarius, R., Smits, D., Gounis, M. J., Leenders, W. P., De Vries, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flow+diverter+implantation+in+a+rat+model+of+sidewall+aneurysm:+a+feasibility+study.">Flow diverter implantation in a rat model of sidewall aneurysm: a feasibility study.</a> J Neurointerv Surg. , (2017).</li><li>Indolfi, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+rat+model+of+small+vessel+stenting.">A new rat model of small vessel stenting.</a> Basic Res Cardiol. 95 (3), 179-185 (2000).</li><li>Oyamada, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trans-iliac+rat+aorta+stenting:+a+novel+high+throughput+preclinical+stent+model+for+restenosis+and+thrombosis.">Trans-iliac rat aorta stenting: a novel high throughput preclinical stent model for restenosis and thrombosis.</a> J Surg Res. 166 (1), e91-e95 (2011).</li><li>Kwon, J. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+bare+metal+stent+and+paclitaxel-eluting+stent+using+a+novel+rat+aorta+stent+model.">Comparison of bare metal stent and paclitaxel-eluting stent using a novel rat aorta stent model.</a> J Vet Sci. 12 (2), 143-149 (2011).</li><li>Rouer, M., Meilhac, O., Delbosc, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+murine+model+of+endovascular+aortic+aneurysm+repair.">A new murine model of endovascular aortic aneurysm repair.</a> J Vis Exp. (77), e50740 (2013).</li><li>Hardhammar, P. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduction+in+thrombotic+events+with+heparin-coated+Palmaz-Schatz+stents+in+normal+porcine+coronary+arteries.">Reduction in thrombotic events with heparin-coated Palmaz-Schatz stents in normal porcine coronary arteries.</a> Circulation. 93 (3), 423-430 (1996).</li><li>Guerrero, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antioxidant+effects+of+a+single+dose+of+acetylsalicylic+acid+and+salicylic+acid+in+rat+brain+slices+subjected+to+oxygen-glucose+deprivation+in+relation+with+its+antiplatelet+effect.">Antioxidant effects of a single dose of acetylsalicylic acid and salicylic acid in rat brain slices subjected to oxygen-glucose deprivation in relation with its antiplatelet effect.</a> Neurosci Lett. 358 (3), 153-156 (2004).</li><li>Niitsu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Repeat+oral+dosing+of+prasugrel,+a+novel+P2Y12+receptor+inhibitor,+results+in+cumulative+and+potent+antiplatelet+and+antithrombotic+activity+in+several+animal+species.">Repeat oral dosing of prasugrel, a novel P2Y12 receptor inhibitor, results in cumulative and potent antiplatelet and antithrombotic activity in several animal species.</a> Eur J Pharmacol. 579 (1-3), 276-282 (2008).</li><li>Kastrati, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracoronary+stenting+and+angiographic+results:+strut+thickness+effect+on+restenosis+outcome+(ISAR-STEREO)+trial.">Intracoronary stenting and angiographic results: strut thickness effect on restenosis outcome (ISAR-STEREO) trial.</a> Circulation. 103 (23), 2816-2821 (2001).</li><li>Lowe, H. C., James, B., Khachigian, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+model+of+in-stent+restenosis:+rat+aortic+stenting.">A novel model of in-stent restenosis: rat aortic stenting.</a> Heart. 91 (3), 393-395 (2005).</li><li>Finn, A. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+rat+model+of+carotid+artery+stenting+for+the+understanding+of+restenosis+in+metabolic+diseases.">A novel rat model of carotid artery stenting for the understanding of restenosis in metabolic diseases.</a> J Vasc Res. 39 (5), 414-425 (2002).</li></ol>

이 작품은 연구 위원회, Kantonsspital 아라우, 아라우, 스위스의 연구 기금에 의해 지원 되었다. 생 분해성 마그네슘 스텐 트 Biotronik AG, 혈관 중재, Buelach, 스위스 센터에 의해 공급 되었다. 저자는 전적으로 디자인 제시 연구의 행위에 대 한 책임 고 아무 경쟁 관심사를 선언 합니다.

Bioabsorbable 스텐 트 및 동물 모델 최근 몇 년 동안에서 의학의 일반적인 경향 영구 임 플 란 트 (는 그들의 삶의 나머지 부분에 대 한 환자의 시체에 남아)에서 바이오 흡수 재료에 되었습니다. 마그네슘 stents, 특히, 이미 상당히 심장8,9에 설치 된다. 불행히도이 텐트는 아직 테스트 되지 뇌혈관 질환 같은 다른 응용 프로그램에 대 한. 이런 ?...

때문에 파열된 intracranial 동맥의 거미 막 밑 출혈은 높은 사망 율 및 빈약한 신경학 상 결과 많은 생존자와 연결. 현재 두 가지 일반적인 방법을 IA를 가리고 하: 어느 microsurgical 클리핑 (이 동맥의 요원 노출 필요), 또는 혈관 내 수술 폐색. 좁은 센 IA의 보다 적게 침략 적 혈관 내 수술 코일 치료 (특히 후부 순환을1,2)에서 약간 낮은 병 적 상태와 관련 된 것으로 표시 되었습니다로 혈관 내 수술 치료 옵션이 되고있다는 많은 신경외과 센터의 선호 적임입니다. 수많은 장치 혈관 내 수술 치료의 표시를 확장 하 고 감기 후 아이오와 재발의 주요 한계를 극복 하기 위해 개발 되었습니다. Intracranial 텐트는 특히 그들은 네오-endothelization에 대 한 비 계 역할 및 헤 르 니 아 예방, 코일로 부모 동맥을 보호 하 고 개선 intraluminal intraaneurysmal 혈전 증으로 인 한 이러한 한계를 극복 하기 위해 유망한 혈액 유입의 감소입니다. 저기 저가 동물 모델; 소설 intracranial 스텐 트를 공부 필요 분자와 거시적인 수준.
이 연구의 목적은 빠르고, 안전 하 고, 디자인 하 고 텐트 쥐3,,45에 이미 설립된 saccular 동맥 모델에서 응용 프로그램에 대 한 수술 기법을 표준화. 현재 프로젝트에서 우리는 생 분해성 마그네슘 텐트의 역할 평가.

<table><tbody><tr><td>Medetomidine</td><td></td><td></td><td>any generic</td></tr><tr><td>Ketamin</td><td></td><td></td><td>any generic</td></tr><tr><td>Buprenorphine</td><td></td><td></td><td>any generic</td></tr><tr><td>Phosphate buffered saline</td><td></td><td></td><td></td></tr><tr><td>Sodium dodecyl sulfate (0.1%)</td><td></td><td></td><td></td></tr><tr><td>3-0 resorbable suture</td><td>Ethicon Inc., USA</td><td>VCP428G</td><td></td></tr><tr><td>5-0 non absorbable suture</td><td>Ethicon Inc., USA</td><td>8618G</td><td></td></tr><tr><td>6-0 non-absorbable suture</td><td>B. Braun, Germany</td><td>C0766070</td><td></td></tr><tr><td>9-0 non-absorbable suture</td><td>B. Braun, Germany</td><td>G1111140</td><td></td></tr><tr><td>10-0 non-absorbable suture</td><td>Covidien, USA</td><td>N2530 Monosof</td><td></td></tr><tr><td>Operation microscope</td><td>Zeiss, Germany</td><td></td><td></td></tr><tr><td>Digital microscope camera</td><td>Sony, Japan</td><td>HXR-MC1P</td><td></td></tr><tr><td>Standard surgical instruments</td><td></td><td>multiple</td><td>see protocol 7.a</td></tr><tr><td>Microsurgical instruments</td><td></td><td>multiple</td><td>see protocol 7.b</td></tr><tr><td>Vascular clip applicator</td><td>B. Braun, Germany</td><td>FT495T</td><td></td></tr><tr><td>Temporary vascular clamps</td><td>B. Braun, Germany</td><td></td><td></td></tr><tr><td>19G Puncture needle&amp;nbsp;</td><td>Angiomed GmbH, Germany</td><td>15820010</td><td></td></tr><tr><td>Hydrophobic guide wire</td><td>Cook Medical, USA</td><td>G00650</td><td></td></tr><tr><td>4F sheat</td><td>Cordis Corporation, USA</td><td>504-604A</td><td></td></tr><tr><td>Inflation syringe</td><td></td><td></td><td></td></tr><tr><td>Laboratory shaker</td><td>Stuart</td><td>SRT6</td><td></td></tr><tr><td>Magnesium Stent 2.5/6 AMS with Polymer coating</td><td>Biotronik, Switzerland</td><td></td><td></td></tr><tr><td>Surgery drape&amp;nbsp;</td><td></td><td></td><td></td></tr><tr><td>Sterile cellulose swabs</td><td></td><td></td><td></td></tr><tr><td>Syringes 1 ml and 2 ml</td><td></td><td></td><td></td></tr><tr><td>Hollow needles 18G and 26G</td><td></td><td></td><td></td></tr><tr><td>Isotonic sodium chloride</td><td></td><td></td><td></td></tr><tr><td>Microtubes</td><td></td><td></td><td></td></tr><tr><td>Eye ointment</td><td>Bausch + Lomb Inc, USA</td><td>Lacrinorm</td><td>any generic</td></tr><tr><td>Small animal shaver</td><td></td><td></td><td></td></tr></tbody></table>

biodegradable magnesium stent treatment of saccular aneurysms in a rat model - introduction of the surgical technique

Intracranial 동맥 류 혈관 내 수술 치료에 사용할 수 있는 기술의 armamentarium에 꾸준한 progess stents 및 흐름 diverters 같은 소설 색전술 재료 테스트 실험 동물 모델을 저렴 하 고 reproducable를 요구 한다. 현재 프로젝트의 목표는 안전 하 고, 빠르고, 디자인 하 고 텐트에 대 한 표준화 된 수술 기법 쥐 동물 모델에서 saccular 동맥 류 색전술을 지원.
Saccular 동맥 류 하강 대동맥에서 동맥 이식에서 창조 되었다. 동맥 류 했다 microsurgically는 syngenic 남성 Wistar 쥐 무게의 infrarenal 복 부 대동맥에 엔드-투-사이드 문 합을 통해 이식 > 500 g. 동맥 문 합, 다음 동맥 류 색전술 풍선 확장 마그네슘 텐트 (2.5 m m x 6 m m)를 사용 하 여 수행 되었다. 텐트 시스템 퇴행 성 변형된 Seldinger 기술을 사용 하 여 더 낮은 복 부 대동맥에서 도입 했다.
6 동물의 파일럿 시리즈, 다음 67 쥐의 총 설립된 표준 운영 절차에 따라 운영 했다. 수술 시간, 말은 문 합 시간, 그리고 의미 했다 167 ± 22 분, 26 ± 동맥 펑크 사이트의 시간을 봉합 뜻 6 분 및 11 ± 5 분, 각각. 사망 율은 6% (n = 4). 병 적 율은 7.5% (n = 5)에 스 텐트 혈전 증 4 경우에 발견 되었다 (n 2 초기, n = = 스 텐트 혈전 증에 2).
결과 saccular sidewall 동맥 류 쥐-병 적 상태와 사망률의 낮은 속도의 표준화 된 텐트 폐색의 가능성을 보여 줍니다. 이 텐트 색전술 절차 결합 텐트의 소설 개념을 공부 하는 기회 또는 흐름 분배기 기반 장치 뿐만 아니라 치유의 분자 양상.

수술의 평균 기간 167 (± 22) 분의 26 (± 6) 분 동맥 생성 및 추가 23 (± 7)에 대 한 필요는 텐트 응용 프로그램 및 arteriotomy (그림 3)의 재건에 필요한 분.
사망, 사망률, 그리고 거시적인 텐트 혈전 증 연구의 기본 끝점을 했다. 정기적인 후속 기간 7 일을 했다 (n = 28), 21 일 (n = 32) 각각. 사망 또는 사건이 병 적 연...

Watch this Scientific Journal Video about Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique at JoVE.com

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique

Reproducable 실험 동물 모델 소설 색전술 재료, 혈관 내 수술 intracranial 동맥 류 (IA)의 폐색을 치료 하도록 설계 되었습니다의 테스트 필요 합니다. 현재 공부 saccular 동맥 류 쥐 동물 모델에서의 텐트를 이용한 색전술에 대 한 안전 하 고 표준화 된 수술 기법을 개발 하는 것을 목표로 합니다.

쥐 모델-수술 방법의 소개에에서 Saccular 동맥 류의 생 분해성 마그네슘 스 텐트 치료

The steady progess in the armamentarium of techniques available for endovascular treatment of intracranial aneurysms requires affordable and reproducable ...

biodegradable-magnesium-stent-treatment-saccular-aneurysms-rat-model

Universidad de Cartagena UDEC

Research

JoVE Journal

Neuroscience

11.0K 조회수.  Kantonsspital Aarau. Reproducable 실험 동물 모델 소설 색전술 재료, 혈관 내 수술 intracranial 동맥 류 (IA)의 폐색을 치료 하도록 설계 되었습니다의 테스트 필요 합니다. 현재 공부 saccular 동맥 류 쥐 동물 모델에서의 텐트를 이용한 색전술에 대 한 안전 하 고 표준화 된 수술 기법을 개발 하는 것을 목표로 합니다.

비디오: Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique

A Murine Model of Stent Implantation in the Carotid Artery for the Study of Restenosis

Despite the considerable progress made in the stent development in the last decades, cardiovascular diseases remain the main cause of death in western countries. Beside the benefits offered by the development of different drug-eluting stents, the coronary revascularization bears also the life-threatening risks of in-stent thrombosis and restenosis. Research on new therapeutic strategies is impaired by the lack of appropriate methods to study stent implantation and restenosis processes. Here, we describe a rapid and accessible procedure of stent implantation in mouse carotid artery, which offers the possibility to study in a convenient way the molecular mechanisms of vessel remodeling and the effects of different drug coatings.

A model of stent implantation in mouse carotid artery is described. Compared to other similar methods, this procedure is very rapid, simple and accessible, offering the possibility to study in a convenient way the vascular wall reaction to different drug-eluting stents and the molecular mechanisms of restenosis.

재 협착의 연구에 대한 경동맥 스텐트 시술의 쥐과 모형

Despite  the considerable progress made in the stent development in the last decades,  cardiovascular diseases remain the main cause of death in western ...

연구

의학

A New Murine Model of Endovascular Aortic Aneurysm Repair

Endovascular aneurysm exclusion is a validated technique to prevent aneurysm rupture. Long-term results highlight technique limitations and new aspects of Abdominal aortic aneurysm (AAA) pathophysiology. There is no abdominal aortic aneurysm endograft exclusion model cheap and reproducible, which would allow deep investigations of AAA before and after treatment. We hereby describe how to induce, and then to exclude with a covered coronary stentgraft an abdominal aortic aneurysm in a rat. The well known elastase induced AAA model was first reported in 19901 in a rat, then described in mice2. Elastin degradation leads to dilation of the aorta with inflammatory infiltration of the abdominal wall and intra luminal thrombus, matching with human AAA. Endovascular exclusion with small covered stentgraft is then performed, excluding any interactions between circulating blood and the aneurysm thrombus. Appropriate exclusion and stentgraft patency is confirmed before euthanasia by an angiography thought the left carotid artery. Partial control of elastase diffusion makes aneurysm shape different for each animal. It is difficult to create an aneurysm, which will allow an appropriate length of aorta below the aneurysm for an easy stentgraft introduction, and with adequate proximal and distal neck to prevent endoleaks. Lots of failure can result to stentgraft introduction which sometimes lead to aorta tear with pain and troubles to stitch it, and endothelial damage with post op aorta thrombosis. Giving aspirin to rats before stentgraft implantation decreases failure rate without major hemorrhage. Clamping time activates neutrophils, endothelium and platelets, and may interfere with biological analysis.

Histological and biochemical modifications after aneurysm endograft exclusion are still unclear. We describe a new model of endograft implantation on a murine aneurysm. Through thrombus analysis with and without persistant circulating blood stream interface, and new endovascular biomaterials evaluation, this model will help to better understand endovascular aneurysm exclusion pathobiology.

혈관 동맥류 수리의 새로운 쥐과 모델

Endovascular aneurysm exclusion is a validated technique to prevent aneurysm rupture. Long-term results highlight technique limitations and new aspects of ...

Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model

Preclinical models of restenosis are essential to unravel the pathophysiological processes that lead to in-stent restenosis and to optimize existing and future drug-eluting stents.
A variety of antibodies and transgenic and knockout strains are available in rats. Consequently, a model for in-stent restenosis in the rat would be convenient for pathobiological and pathophysiological studies. 
In this video, we present the full procedure and pit-falls of a rat stent model suitable for high throughput stent research. We will show the surgical procedure of stent deployment, and the assessment of in-stent restenosis using the most elegant technique of OCT (Optical Coherence Tomography). This technique provides high accuracy in assessing plaque CSAs (cross section areas) and correlates well with histological sections, which require special and time consuming embedding and sectioning techniques. OCT imaging further allows longitudinal monitoring of the development of in-stent restenosis within the same animal compared to one-time snapshots using histology.

This video demonstrates how to use a preclinical inexpensive and reliable model to study pathobiological and pathophysiological processes of in-stent restenosis development. Longitudinal in vivo monitoring using OCT (Optical Coherence Tomography) and analysis of OCT images are also demonstrated.

이미징에서 스텐트 - Restenosis : 저렴한, 신뢰성 및 빠른 잠복기 모델

Preclinical models of restenosis are essential to unravel the pathophysiological processes that lead to in-stent restenosis and to optimize existing and ...

생물학

The Helsinki Rat Microsurgical Sidewall Aneurysm Model

Experimental saccular aneurysm models are necessary for testing novel surgical and endovascular treatment options and devices before they are introduced into clinical practice. Furthermore, experimental models are needed to elucidate the complex aneurysm biology leading to rupture of saccular aneurysms.
Several different kinds of experimental models for saccular aneurysms have been established in different species. Many of them, however, require special skills, expensive equipment, or special environments, which limits their widespread use. A simple, robust, and inexpensive experimental model is needed as a standardized tool that can be used in a standardized manner in various institutions.
The microsurgical rat abdominal aortic sidewall aneurysm model combines the possibility to study both novel endovascular treatment strategies and the molecular basis of aneurysm biology in a standardized and inexpensive manner. Standardized grafts by means of shape, size, and geometry are harvested from a donor rat&#39;s descending thoracic aorta and then transplanted to a syngenic recipient rat. The aneurysms are sutured end-to-side with continuous or interrupted 9-0 nylon sutures to the infrarenal abdominal aorta.
We present step-by-step procedural instructions, information on necessary equipment, and discuss important anatomical and surgical details for successful microsurgical creation of an abdominal aortic sidewall aneurysm in the rat.

Microsurgical sidewall aneurysms in rats are created by end-to-side anastomosis of an aortic graft to the abdominal aorta. We present step-by-step instructions and discuss anatomical and surgical details for successful experimental saccular aneurysm creation.

헬싱키 쥐 미세 측벽 동맥류 모델

Experimental saccular aneurysm models are necessary for testing novel surgical and endovascular treatment options and devices before they are introduced ...

Use of a Rat Model to Study Ventral Abdominal Hernia Repair

Ventral abdominal hernia is a relatively common clinical condition that sometimes requires herniorraphy (surgical repair). The repair of ventral abdominal hernia typically requires implantation of a material to serve as a mechanical bridge across the defect in the abdominal wall. Biomaterials, such as porcine small intestinal submucosa (SIS), also serve as a lattice for cell growth into the implant and can naturally incorporate into the host tissue. Development of such repair materials benefits from use of animal models in which experimental abdominal wall defects are easily created and are amenable to repair in a reproducible fashion. The method offered here describes surgical creation and repair of ventral abdominal hernia in a rat model. When SIS is used to repair an experimental ventral abdominal hernia in this model, it is rapidly incorporated into host tissue within 28 days of implantation. Histologically, incorporation of their implanted material into host tissue is characterized by a robust fibrovascular response. Future refinements and applications of the rat abdominal hernia model may likely involve diabetic and/or obese animals as a means to more closely mimic common co-morbidities of man.

This manuscript describes methods associated with creation and repair of a ventral abdominal wall defect (hernia). This model can be used to study repair strategies such as those that use implanted materials. In this manuscript, repair of the experimental hernia with porcine small intestinal submucosa is presented as an example.

복 부 복 부 헤 르 니 아 수리 공부 쥐 모델의 사용

Ventral abdominal hernia is a relatively common clinical condition that sometimes requires herniorraphy (surgical repair). The repair of ventral abdominal ...

생체공학

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

In-stent restenosis presents a major complication of stent-based revascularization procedures widely used to re-establish blood flow through critically narrowed segments of coronary and peripheral arteries. Endovascular stents capable of tunable release of genes with anti-restenotic activity may present an alternative strategy to presently used drug-eluting stents. In order to attain clinical translation, gene-eluting stents must exhibit predictable kinetics of stent-immobilized gene vector release and site-specific transduction of vasculature, while avoiding an excessive inflammatory response typically associated with the polymer coatings used for physical entrapment of the vector. This paper describes a detailed methodology for coatless tethering of adenoviral gene vectors to stents based on a reversible binding of the adenoviral particles to polyallylamine bisphosphonate (PABT)-modified stainless steel surface via hydrolysable cross-linkers (HC). A family of bifunctional (amine- and thiol-reactive) HC with an average t1/2 of the in-chain ester hydrolysis ranging between 5 and 50 days were used to link the vector with the stent. The vector immobilization procedure is typically carried out within 9 hr&#160;and consists of several steps: 1) incubation of the metal samples in an aqueous solution of PABT (4 hr); 2) deprotection of thiol groups installed in PABT with tris(2-carboxyethyl) phosphine (20 min); 3) expansion of thiol reactive capacity of the metal surface by reacting the samples with polyethyleneimine derivatized with pyridyldithio (PDT) groups (2 hr); 4) conversion of PDT groups to thiols with dithiothreitol (10 min); 5) modification of adenoviruses with HC (1 hr); 6) purification of modified adenoviral particles by size-exclusion column chromatography (15 min) and 7) immobilization of thiol-reactive adenoviral particles on the thiolated steel surface (1 hr). This technique has wide potential applicability beyond stents, by facilitating surface engineering of bioprosthetic devices to enhance their biocompatibility through the substrate-mediated gene delivery to the cells interfacing the implanted foreign material.

These studies report on reversible attachment of adenoviral gene vectors to coatless metal surfaces of stents and model mesh disks. Sustained release of transduction-competent viral particles contingent upon hydrolysis of cross-linkers used for vector immobilization results in a durable site-specific transgene expression in vascular cells and in stented arteries.

가수 분해 크로스 링커를 통해 아데노 바이러스 벡터 닿는를 사용하여 금속 스텐​​트 표면에서 혈관 유전자 전송

In-stent restenosis presents a major complication of stent-based revascularization procedures widely used to re-establish blood flow through critically ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic aneurysms (AAAs). This article introduces and describes a standard protocol used to induce AAAs through adventitial VV hypoperfusion created with a combination of polyurethane catheter insertion into the aortic lumen and suture ligation of the infrarenal abdominal aorta.
The protocol involves the use of male rats weighing 300-400 g, which are provided food and water ad libitum. After laparotomy with a ventral midline abdominal incision, exfoliation of the aorta is performed, which blocks blood flow from the perivascular tissue. Aortotomy involving a small incision adjacent to the renal artery branches is performed, and a polyurethane catheter is inserted using an 18-gauge indwelling needle. After repairing the incision, tight ligation of the aorta over the catheter blocks VV blood flow from the proximal direction through the aortic wall without disturbing the aortic blood flow. This technique can induce an AAA with progressive aortic dilatation.
The greatest benefit of this model is that VV hypoperfusion causes tissue hypoxia and the development of an infrarenal AAA, which has morphological and pathological characteristics similar to those of a human AAA.

Polyurethane catheter insertion into the aortic lumen and suture ligation of the aorta induce chronic hypoxia due to hypoperfusion of the adventitial vasa vasorum. This article describes a novel animal model of abdominal aortic aneurysm (AAA) with characteristics similar to those of AAA in humans.

Adventitial 바사 Vasorum 관류를 차단 하 여 복 부 대동맥 동맥 류의 설치류 모델의 생성

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep learning curve. The introduction of the cuff technique for anastomosis of the portal vein (PV) and infrahepatic vena cava (IHVC) has significantly simplified the transplant procedure in rats. However, due to the short anterior wall of the recipients' suprahepatic vena cava (SHVC), the cuff technique is very difficult to use for the reconstruction of the SHVC. Most researchers in this field still use the hand-suture technique for SHVC reconstruction, which makes it the bottleneck step in rat orthotopic liver transplantation. The magnetic anastomosis technique (i.e., magnamosis) is a method of connecting two vessels using the attractive force between two magnets. Our recent study has shown that the magnetic anastomosis technique is superior to the hand-suture technique for SHVC reconstruction in rats. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using the novel magnetic anastomosis technique. In this model, the reconstruction of the PV and IHVC was performed by the standard cuff technique, while the reconstruction of the bile duct (BD) was performed by a stent technique. The hepatic re-arterialization was not performed. The magnetic anastomosis technique made SHVC reconstruction much easier and significantly shortened the anphepatic phase. After a reasonable learning curve, even researchers without advanced microsurgical skills can produce reliable and reproducible results using this rat model of OLT.

The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.

Orthotopic의 쥐 모델 간 이식 Suprahepatic 베 나 정 맥 재건에 대 한 새로운 자석 문 합 기술을 사용 하 여

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep ...

Implantation of Human-Sized Coronary Stents into Rat Abdominal Aorta Using a Trans-Femoral Access

Percutaneous coronary intervention (PCI), combined with the deployment of a coronary stent, represents the gold standard in interventional treatment of coronary artery disease. In-stent restenosis (ISR) is determined by an excessive proliferation of neointimal tissue within the stent and limits the long-term success of stents. A variety of animal models have been used to elucidate pathophysiological processes underlying in-stent restenosis (ISR), with the porcine coronary and the rabbit iliac artery models being the most frequently used. Murine models provide the advantages of high throughput, ease of handling and housing, reproducibility, and a broad availability of molecular markers. The apolipoprotein E deficient (apoE-/- ) mouse model has been widely used to study cardiovascular diseases. However, stents must be miniaturized to be implanted into mice, involving important changes of their mechanical and (potentially) biological properties. The use of apoE-/- rats can overcome these shortcomings as apoE-/- rats allow for the evaluation of human-sized coronary stents while at the same time providing an atherogenic phenotype. This makes them an excellent and reliable model to investigate ISR after stent implantation. Here, we describe, in detail, the implantation of commercially available human coronary stents into the abdominal aorta of rats with an apoE-/- background using a trans-femoral access.

This protocol describes the implantation of human coronary stents into the abdominal aorta of rats with an apoE-/- background using a trans-femoral access. Compared with other animal models, murine models carry the advantages of high throughput, reproducibility, ease of handling and housing, and a broad availability of molecular markers.

대퇴동맥 접근을 이용한 쥐 복부 대동맥에 인간 크기의 관상 동맥 스텐트를 이식

Percutaneous coronary intervention (PCI), combined with the deployment of a coronary stent, represents the gold standard in interventional treatment of ...

Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model

Microsurgical clipping creates a subsequent barrier of blood flow into intracranial aneurysms, whereas endovascular treatment relies on neointima and thrombus formation. The source of endothelial cells covering the endoluminal layer of the neointima remains unclear. Therefore, the aim of the present study was to investigate the origin of neointima-forming cells after cell-tracer injection in the already well-established Helsinki rat microsurgical sidewall aneurysm model.
Sidewall aneurysms were created by suturing decellularized or vital arterial pouches end-to-side to the aorta in male Lewis rats. Before arteriotomy with aneurysm suture, a cell-tracer injection containing CM-Dil dye was performed into the clamped aorta to label endothelial cells in the adjacent vessel and track their proliferation during follow-up (FU). Treatment followed by coiling (n = 16) or stenting (n = 15). At FU (7 days or 21 days), all rats underwent fluorescence angiography, followed by aneurysm harvesting and macroscopic and histological evaluation with immunohistological cell counts for specific regions of interest. 
None of the 31 aneurysms had ruptured upon follow-up. Four animals died prematurely. Macroscopically residual perfusion was observed in 75.0% coiled and 7.0% of stented rats. The amount of cell-tracer-positive cells was significantly elevated in decellularized stented compared to coiled aneurysms with respect to thrombus on day 7 (p = 0.01) and neointima on day 21 (p = 0.04). No significant differences were found in thrombus or neointima in vital aneurysms. 
These findings confirm worse healing patterns in coiled compared to stented aneurysms. Neointima formation seems particularly dependent on the parent artery in decellularized aneurysms, whereas it is supported by the recruitment from aneurysm wall cells in vital cell-rich walls. In terms of translation, stent treatment might be more appropriate for highly degenerated aneurysms, whereas coiling alone might be adequate for aneurysms with mostly healthy vessel walls.

We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.

세포 추적기 주입을 사용하여 쥐 saccular 측벽 모델에서 Neointima-Forming Cells의 기원을 조사합니다.

Microsurgical clipping creates a subsequent barrier of blood flow into intracranial aneurysms, whereas endovascular treatment relies on neointima and ...

쥐 모델-수술 방법의 소개에에서 Saccular 동맥 류의 생 분해성 마그네슘 스 텐트 치료

요약

더 많은 비디오 탐색

이 비디오의 챕터

재 협착의 연구에 대한 경동맥 스텐트 시술의 쥐과 모형

혈관 동맥류 수리의 새로운 쥐과 모델

이미징에서 스텐트 - Restenosis : 저렴한, 신뢰성 및 빠른 잠복기 모델

헬싱키 쥐 미세 측벽 동맥류 모델

복 부 복 부 헤 르 니 아 수리 공부 쥐 모델의 사용

가수 분해 크로스 링커를 통해 아데노 바이러스 벡터 닿는를 사용하여 금속 스텐트 표면에서 혈관 유전자 전송

Adventitial 바사 Vasorum 관류를 차단 하 여 복 부 대동맥 동맥 류의 설치류 모델의 생성

Orthotopic의 쥐 모델 간 이식 Suprahepatic 베 나 정 맥 재건에 대 한 새로운 자석 문 합 기술을 사용 하 여

대퇴동맥 접근을 이용한 쥐 복부 대동맥에 인간 크기의 관상 동맥 스텐트를 이식

세포 추적기 주입을 사용하여 쥐 saccular 측벽 모델에서 Neointima-Forming Cells의 기원을 조사합니다.

쥐 모델-수술 방법의 소개에에서 Saccular 동맥 류의 생 분해성 마그네슘 스 텐트 치료

요약

더 많은 비디오 탐색

이 비디오의 챕터

재 협착의 연구에 대한 경동맥 스텐트 시술의 쥐과 모형

혈관 동맥류 수리의 새로운 쥐과 모델

이미징에서 스텐트 - Restenosis : 저렴한, 신뢰성 및 빠른 잠복기 모델

헬싱키 쥐 미세 측벽 동맥류 모델

복 부 복 부 헤 르 니 아 수리 공부 쥐 모델의 사용

가수 분해 크로스 링커를 통해 아데노 바이러스 벡터 닿는를 사용하여 금속 스텐​​트 표면에서 혈관 유전자 전송

Adventitial 바사 Vasorum 관류를 차단 하 여 복 부 대동맥 동맥 류의 설치류 모델의 생성

Orthotopic의 쥐 모델 간 이식 Suprahepatic 베 나 정 맥 재건에 대 한 새로운 자석 문 합 기술을 사용 하 여

대퇴동맥 접근을 이용한 쥐 복부 대동맥에 인간 크기의 관상 동맥 스텐트를 이식

세포 추적기 주입을 사용하여 쥐 saccular 측벽 모델에서 Neointima-Forming Cells의 기원을 조사합니다.

가수 분해 크로스 링커를 통해 아데노 바이러스 벡터 닿는를 사용하여 금속 스텐트 표면에서 혈관 유전자 전송