太平洋の北西国立研究所 (なり) 核プロセス科学イニシアティブ (NPSI) 感謝しております-支援のため監督研究と開発 (LDRD) 基金。・ チャタジー博士 Sayandev は、合成ベーマイト粒子を提供されています。インストゥルメンタル アクセスが w. r. ワイリー環境分子科学研究所 (EMSL) 一般ユーザーの提案書を提供されました。EMSL はオフィスの生物と環境研究 (BER) なりで主催国立科学ユーザー施設です。バテル契約 DE AC05 76RL01830 下 DOE のなりが運営しています。

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著者が明らかに何もありません。

SEM は、高分解能1ナノ (nm) レベルの有機・無機材料における表面キャラクタリゼーションの強力な手法です。たとえば、地質試料26と半導体27などしっかりと乾燥試料を解析する広く使用されます。しかし、電子顕微鏡1に必要な高真空環境の中で液体の不適合による湿式、液体のサンプルを特徴付けることの制限がありま...

走査型電子顕微鏡 (SEM) は、高解像度イメージング1を生産することによって様々 な標本の調査に広く適用されています。エネルギー分散型 x 線分光 (EDX)、SEM に関連付けられているは、元素組成1の決定を可能。 にします。伝統的に、イメージングのだけ乾燥した固体サンプルの SEM が適用されます。最後の 30 年で環境 SEM (フラッディングガス) は、蒸気環境2,3,4、5の部分的な水和物サンプルを分析するため開発されました。ただし、フラッディングガスは目的の高解像度6でウェット、完全流体のサンプルをイメージできません。ウェットの SEM 細胞も濡れている画像試料の SEM7,8; を使用して開発されました。それにもかかわらず、これらの細胞が電子イメージングを後方散乱し、それらデザイン9,10アプリケーションのアクセシビリティの高いに生体試料を中心に開発されました。
SEM を使用して、ネイティブの液体環境の様々 なサンプルを分析する際の課題に対処するため、我々 は真空対応マイクロ流体デバイス、分析で、液体真空インターフェイス (サルヴィ)、高空間分解能セカンダリを有効にするためのシステムを発明しました。SEM の高真空モードを用いた液体試料の電子 (SE) イメージングと元素分析この手法には、次のユニークな機能が含まれています: 1) 液体が直接では、直径 1-2 マイクロメートルの小さな開口部でプローブ2) 液体は表面張力によって穴内に保持されます。・ 3) サルヴィはポータブルで、1 つ以上分析プラットフォーム11,12,13,14,15,16,17 に適応することができます ,18。
サルヴィは 100 nm 厚のシリコン窒化 (罪) 膜で構成されています、200 μ m の広いマイクロ製ポリジメチルシロキサン (PDMS) ブロック。罪膜ウィンドウは、マイクロをシールに適用されます。作製の詳細と重要な設計上の考慮事項は、以前の論文と特許11,19,20詳細いた。現在、大手メーカーと顕微鏡用消耗品の供給のディストリビューターは、液体 SEM アプリケーション21,22の市販サルヴィ デバイスを販売するためのライセンスを購入しています。
分析機器の真空ベースのサルヴィのアプリケーションは、さまざまな水溶液とバイオ フィルム、哺乳類細胞、ナノ粒子、および電極材料12,を含む複雑な液体混合物を使用して実証されています。14,17,20,23,24します。 ただし、前述の仕事のほとんどは従って液体のアプリケーション キー分析ツールとして飛行時間二次イオン質量分析 (TOF-SIMS) を利用サルヴィの SEM が十分に検討されていません。今回、サルヴィは液体の SEM 画像と EDX 元素分析を用いた液体の大きい非球状粒子の研究に使用されています。サンプルは研究室で合成された AlOOH 粒子から成っています。サブマイクロ メーター サイズ ベーマイト粒子は、ハンフォード ・ サイトで高レベル放射性廃棄物に存在する知られています。溶解する遅く、廃棄物処理にレオロジーの問題を引き起こす可能性があります。したがって、液体25ベーマイト粒子を特徴付ける機能を持つことが重要です。この技術的なアプローチは、これらの粒子と関連のレオロジー特性の改善された理解の様々 な物理化学的条件のベーマイトを研究に使用できます。これらの粒子は、液体中に浮遊する粒子を研究するために高真空 SEM にサルヴィを適用する方法を示すステップバイ ステップを利用しました。サルヴィと SEM の統合および SEM データ集録のための重要な技術的なポイントは、用紙内で強調表示されます。
プロトコルは、将来的に液体の SEM の多様なアプリケーションでこの手法を活用に興味がある人のためサルヴィと液体の SEM イメージングを用いた液体試料解析のデモを提供します。

<table><tbody><tr><td>Carbon Coater</td><td>Cressington</td><td>208 Carbon</td><td>It is accompanied with thickness monitor MTM-10.</td></tr><tr><td>SEM</td><td>FEI</td><td>Quanta 3D FEG</td><td>It provides highly resolved scanning electron microscopy and elemental analysis.</td></tr><tr><td>System for Analysis at the Liquid Vacuum Interface (SALVI)</td><td>Pacific Northwest National Laboratory</td><td>N/A</td><td>SALVI is a unique, vacuum compatible microfluidic cell that enables the characterization of the liquid sample using vacuu- based scientific instrument.</td></tr><tr><td>PEEK Union</td><td>Valco</td><td>ZU1TPK</td><td>The polyether ether ketone union is used for connecting the inlet and outlet of SALVI</td></tr><tr><td>Syringe</td><td>BD</td><td>309659</td><td>1 mL</td></tr><tr><td>Pipette</td><td>Thermo Fisher Scientific</td><td>21-377-821</td><td>Range: 100 to 1,000 mL</td></tr><tr><td>Pipette Tip 1</td><td>Neptune</td><td>2112.96.BS</td><td>1,000 &amp;micro;L</td></tr><tr><td>Pipette Tip 2</td><td>Rainin</td><td>17001865</td><td>20 &amp;micro;L</td></tr><tr><td>Syringe Pump</td><td>Harvard Apparatus</td><td>70-2213</td><td>It is used to inject the liquid sample into the SALVI device.</td></tr><tr><td>pH meter</td><td>Fisher Scientific/accumet</td><td>13-636-AP72</td><td>It is used for measuring the pH of AlOOH in DI water.</td></tr><tr><td>Barnstead Ultrapure Water System, UV/UF</td><td>Thermo Scientific Barnstead</td><td>Nanopure diamond D11931</td><td>It is used for producing DI water.</td></tr><tr><td>Centrifuge tubes</td><td>Fisher scientific/Falcon</td><td>15-527-90</td><td>15 mL</td></tr><tr><td>Bransonic ultrasonic cleaner</td><td>Sigma-Aldrich</td><td>2510</td><td>It is used to ultrasonicate the AlOOH liquid sample.</td></tr><tr><td>Balance</td><td>Mettler Toledo</td><td>11106015</td><td>XS64</td></tr><tr><td>AlOOH</td><td>Pacific Northwest National Laboratory</td><td>N/A</td><td>It is synthesized by scientists at Pacific Northwest National Laboratory.</td></tr><tr><td>xT microscope Control</td><td>FEI</td><td>Quanta 3D FEG</td><td>Default microscope control software of SEM Quanta 3D FEG</td></tr><tr><td>EDAX Genesis software</td><td>EDAX</td><td>N/A</td><td>The software is used for collecting the EDX elemental information of the samples.</td></tr><tr><td>Teflon tubing</td><td>SUPELCO</td><td>58697-U</td><td>It is used for introducing the sample into the microchannel and holding adequate volume of liquid.</td></tr></tbody></table>

in situ characterization of boehmite particles in water using liquid sem

液体真空インターフェイス (サルヴィ) と走査型電子顕微鏡 (SEM) での解析システムを用いたin situイメージングおよび元素ベーマイト (AlOOH) 中の異物分析水を実現します。本稿では、法と真空を統合することの重要なステップ SEM と二次電子の高真空下での液体中の粒子の (SE) 画像を得るに互換性のある SAVLI。エネルギー分散型 x 線分光 (EDX) を使用 (DI) 純水のみと同様空のチャネルを含む液とコントロールのサンプル中の粒子の元素分析を取得します。液中粒子合成ベーマイト (AlOOH) は、液体の SEM 図にモデルとして使用されます。結果は良好な分解能を持つ SE モードで粒子がイメージ化されることを示す (すなわち、400 nm)。AlOOH EDX スペクトルは、アルミニウム (Al) ・ ディ ・水と比較すると、空のチャネル コントロールから重要な信号を示しています。液体 SEMその場では、多くのエキサイティングなアプリケーションと液体中の粒子を研究する強力な手法です。この手順は、液体の SEM 画像とサルヴィを用いた EDX 分析を実施するために、このアプローチを使用する場合、潜在的な落とし穴を減らすために技術的なノウハウを提供するために目指しています。

粒子のイメージを作成する方法を示す代表的な結果を示す EDX と相まって液体 SEM 画像その場を用いた解析と。結果には、SE の画像と EDX スペクトルがあります。100,000 X と図 1に 200,000 X 倍率で SE の画像を得た。図 1 aは、AlOOH の SE のイメージを示しています図 1 b ・ ディ ・水と?...

Watch this Scientific Journal Video about In Situ Characterization of Boehmite Particles in Water Using Liquid SEM at JoVE.com

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

その場で液体の SEM を用いた水中のベーマイト粒子のキャラクタリゼーション

その場で液体走査型電子顕微鏡によってリアルタイム イメージングおよび脱イオン水にベーマイト粒子の元素組成分析の手順を紹介します。

In situ imaging and elemental analysis of boehmite (AlOOH) particles in water is realized using the System for Analysis at the Liquid Vacuum Interface ...

in-situ-characterization-boehmite-particles-water-using-liquid

Universidad de Cartagena UDEC

Research

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Chemistry

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

9.1K ビュー.  Pacific Northwest National Laboratory. その場で液体走査型電子顕微鏡によってリアルタイム イメージングおよび脱イオン水にベーマイト粒子の元素組成分析の手順を紹介します。

ビデオ: In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Simulation of the Planetary Interior Differentiation Processes in the Laboratory

A planetary interior is under high-pressure and high-temperature conditions and it has a layered structure. There are two important processes that led to that layered structure, (1) percolation of liquid metal in a solid silicate matrix by planet differentiation, and (2) inner core crystallization by subsequent planet cooling. We conduct high-pressure and high-temperature experiments to simulate both processes in the laboratory. Formation of percolative planetary core depends on the efficiency of melt percolation, which is controlled by the dihedral (wetting) angle. The percolation simulation includes heating the sample at high pressure to a target temperature at which iron-sulfur alloy is molten while the silicate remains solid, and then determining the true dihedral angle to evaluate the style of liquid migration in a crystalline matrix by 3D visualization. The 3D volume rendering is achieved by slicing the recovered sample with a focused ion beam (FIB) and taking SEM image of each slice with a FIB/SEM crossbeam instrument. The second set of experiments is designed to understand the inner core crystallization and element distribution between the liquid outer core and solid inner core by determining the melting temperature and element partitioning at high pressure. The melting experiments are conducted in the multi-anvil apparatus up to 27 GPa and extended to higher pressure in the diamond-anvil cell with laser-heating. We have developed techniques to recover small heated samples by precision FIB milling and obtain high-resolution images of the laser-heated spot that show melting texture at high pressure. By analyzing the chemical compositions of the coexisting liquid and solid phases, we precisely determine the liquidus curve, providing necessary data to understand the inner core crystallization process.

The high-pressure and high-temperature experiments described here mimic planet interior differentiation processes. The processes are visualized and better understood by high-resolution 3D imaging and quantitative chemical analysis.

研究室での惑星インテリア分化過程のシミュレーション

A planetary interior is under high-pressure and high-temperature conditions and it has a layered structure. There are two important processes that led to ...

工学

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Element diffusion rates and temperature/pressure control a range of fundamental volcanic and metamorphic processes. Such processes are often recorded in lamellae exsolved from host mineral phases. Thus, the analysis of the orientation, size, morphology, composition and spacing of exsolution lamellae is an area of active research in the geosciences. The conventional study of these lamellae has been conducted by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and more recently with focused ion beam (FIB)-based nanotomography, yet with limited chemical information. Here, we explore the use of atom probe tomography (APT) for the nanoscale analysis of ilmenite exsolution lamellae in igneous titanomagnetite from ash deposits erupted from the active Soufri&#232;re Hills Volcano (Montserrat, British West Indies). APT allows the precise calculation of interlamellar spacings (14&#8211;29 &#177; 2 nm) and reveals smooth diffusion profiles with no sharp phase boundaries during the exchange of Fe and Ti/O between the exsolved lamellae and the host crystal. Our results suggest that this novel approach permits nanoscale measurements of lamellae composition and interlamellar spacing that may provide a means to estimate the lava dome temperatures necessary to model extrusion rates and lava dome failure, both of which play a key role in volcanic hazard mitigation efforts.

Analysis of the morphology, composition, and spacing of exsolution lamellae can provide essential information to understand geological processes related to volcanism and metamorphism. We present a novel application of APT for the characterization of such lamellae and compare this approach to the conventional use of electron microscopy and FIB-based nanotomography.

エクスブソリュート鉱物相の原子プローブ断層撮影解析

Element diffusion rates and temperature/pressure control a range of fundamental volcanic and metamorphic processes. Such processes are often recorded in ...

環境学

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory

Given their potential for significant property improvements relative to their large grained counterparts, much work has been devoted to the continued development of nanocrystalline metals. Despite these efforts, the transition of these materials from the lab bench to actual applications has been blocked by the inability to produce large scale parts that retain the desired nanocrystalline microstructures. Following the development of a method proven to stabilize the nanosized grain structure to temperatures approaching that of the melting point for the given metal, the US Army Research Laboratory (ARL) has progressed to the next stage in the development of these materials - namely the production of large scale parts suitable for testing and evaluation in a range of relevant test environments. This report provides a broad overview of the ongoing efforts in the processing, characterization, and consolidation of these materials at ARL. In particular, focus is placed on the methodology used for producing the nanocrystalline metal powders, in both small and large-scale amounts, that are at the center of ongoing research efforts.

This paper provides a brief overview of the ongoing efforts at the Army Research Laboratory on the processing of bulk nanocrystalline metals with an emphasis on the methodologies used for the production of the novel metal powders.

米国陸軍研究所で一括ナノ多結晶金属の処理

Given their potential for significant property improvements relative to their large grained counterparts, much work has been devoted to the continued ...

Determination of Aggregate Surface Morphology at the Interfacial Transition Zone (ITZ)

Here, we present a comprehensive method to illustrate the uneven distribution of the interfacial transition zone (ITZ) around the aggregate and the effect of aggregate surface morphology on the formation of ITZ. First, a model concrete sample is prepared with a spherical ceramic particle in roughly the central part of the cement matrix, acting as a coarse aggregate used in common concrete/mortar. After curing until the designed age, the sample is scanned by X-ray computed tomography to determine the relative location of the ceramic particle inside the cement matrix. Three locations of the ITZ are chosen: above the aggregate, on the side of the aggregate, and below the aggregate. After a series of treatments, the samples are scanned with a SEM-BSE detector. The resultant images were further processed using a digital image processing method (DIP) to obtain quantitative characteristics of the ITZ. The surface morphology is characterized at the pixel level based on the digital image. Thereafter, K-means clustering method is used to illustrate the effect of surface roughness on ITZ formation.

Determination of Aggregate Surface Morphology at the Interfacial Transition Zone ITZ

Hereby, we proposed a protocol to illustrate the effect of aggregate surface morphology on the ITZ microstructure. The SEM-BSE image were quantitatively analyzed to obtain ITZ's porosity gradient via digital image processing and a K-means clustering algorithm was further employed to establish a relationship between porosity gradient and surface roughness.

界面遷移帯における凝集表面形態の測定(ITZ)

Here, we present a comprehensive method to illustrate the uneven distribution of the interfacial transition zone (ITZ) around the aggregate and the effect ...

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy

Physical and chemical processes at solid-liquid interfaces play a crucial role in many natural and technological phenomena, including catalysis, solar energy and fuel generation, and electrochemical energy storage. Nanoscale characterization of such interfaces has recently been achieved using cryogenic electron microscopy, thereby providing a new path to advancing our fundamental understanding of interface processes.
This contribution provides a practical guide to mapping the structure and chemistry of solid-liquid interfaces in materials and devices using an integrated cryogenic electron microscopy approach. In this approach, we pair cryogenic sample preparation which allows stabilization of solid-liquid interfaces with cryogenic focused ion beam (cryo-FIB) milling to create cross-sections through these complex buried structures. Cryogenic scanning electron microscopy (cryo-SEM) techniques performed in a dual-beam FIB/SEM enable direct imaging as well as chemical mapping at the nanoscale. We discuss practical challenges, strategies to overcome them, as well as protocols for obtaining optimal results. While we focus in our discussion on interfaces in energy storage devices, the methods outlined are broadly applicable to a range of fields where solid-liquid interface play a key role.

Cryogenic Focused Ion Beam (FIB) and Scanning Electron Microscopy (SEM) techniques can provide key insights into the chemistry and morphology of intact solid-liquid interfaces. Methods for preparing high quality Energy Dispersive X-ray (EDX) spectroscopic maps of such interfaces are detailed, with a focus on energy storage devices.

クライオ集束イオンビームフライス加工と走査型電子顕微鏡・分光法の結合による液体-固体界面のナノスケール特性評価

Physical and chemical processes at solid-liquid interfaces play a crucial role in many natural and technological phenomena, including catalysis, solar ...

<meta charset="utf8"></head><body>外植片モデルを用いた骨形成のマルチモーダル解析

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

The extensive characterization of tissue mineralization in the context of bone regeneration represents a significant challenge, given the numerous modalities that are currently available for analysis. Here, we propose a workflow for a comprehensive evaluation of new bone formation using a relevant large animal osseous ex vivo explant. A bone defect (diameter = 3.75 mm; depth = 5.0 mm) is created in an explanted sheep femoral head and injected with a macroporous bone substitute loaded with a pro-osteogenic growth factor (bone morphogenetic protein 2 - BMP2). Subsequently, the explant is maintained in culture for a 28-day period, allowing cellular colonization and subsequent bone formation. To evaluate the quality and structure of newly mineralized tissue, the following successive methods are set up: (i) Characterization and high-resolution 3D images of the entire explant using micro-CT, followed by deep learning image analyses to enhance the discrimination of mineralized tissues; (ii) Nano-indentation to determine the mechanical properties of the newly formed tissue; (iii) Histological examinations, such as Hematoxylin/Eosin/Saffron (HES), Goldner's trichrome, and Movat's pentachrome to provide a qualitative assessment of mineralized tissue, particularly with regard to the visualization of the osteoid barrier and the presence of bone cells; (iv) Back-scattering scanning electron microscopy (SEM) mapping with internal reference to quantify the degree of mineralization and provide detailed insights into surface morphology, mineral composition, and bone-biomaterial interface; (v) Raman spectroscopy to characterize the molecular composition of the mineralized matrix and to provide insights into the persistence of BMP2 within the cement through the detection of peptide bonds. This multimodal analysis will provide an effective assessment of newly formed bone and comprehensive qualitative and quantitative insights into mineralized tissues. Through the standardization of these protocols, we aim to facilitate interstudy comparisons and improve the validity and reliability of research findings.

<meta charset="utf8"></head><body>著者スポットライト:骨再生研究における組織石灰化の特性評価のための高度な技術

The proposed protocol entails a global approach to assess bone formation in the context of bone regeneration using multimodal analyses. It aims to provide qualitative and quantitative information on new bone formation, enhancing the rigor and validity of basic and pre-clinical investigations.

Ex Vivoモデルにおける組織石灰化の包括的な特性評価

The extensive characterization of tissue mineralization in the context of bone regeneration represents a significant challenge, given the numerous ...

生物工学

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Characterizing the first event of biological production of calcium carbonate requires a combination of microscopy approaches. First, intracellular pH distribution and calcium ions can be observed using live microscopy over time. This allows identification of the life stage and the tissue with the feature of interest for further electron microscopy studies. Life stage and tissues of interest are typically higher in pH and Ca signals. 
Here, using H. elegans, we present a protocol to characterize the presence of calcium carbonate structures in a biological specimen on the scanning electron microscope (SEM), using energy-dispersive X-ray spectroscopy (EDS) to visualize elemental composition, using electron backscatter diffraction (EBSD) to determine the presence of crystalline structures, and using transmission electron microscopy (TEM) to analyze the composition and structure of the material. In this protocol, a focused ion beam (FIB) is used to isolate samples with dimension suitable for TEM analysis. As FIB is a site specific technique, we demonstrate how information from the previous techniques can be used to identify the region of interest, where Ca signals are highest.

We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.

マリンTubewormでライブ光学および電子顕微鏡技術を用いた石灰化イベントのキャラクタリゼーション

Characterizing the first event of biological production of calcium carbonate requires a combination of microscopy approaches. First, intracellular pH ...

生物学

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Membrane remodeling occurs constantly at the plasma membrane and within cellular organelles. To fully dissect the role of the environment (ionic conditions, protein and lipid compositions, membrane curvature) and the different partners associated with specific membrane reshaping processes, we undertake in vitro bottom-up approaches. In recent years, there has been keen interest in revealing the role of septin proteins associated with major diseases. Septins are essential and ubiquitous cytoskeletal proteins that interact with the plasma membrane. They are implicated in cell division, cell motility, neuro-morphogenesis, and spermiogenesis, among other functions. It is, therefore, important to understand how septins interact and organize at membranes to subsequently induce membrane deformations and how they can be sensitive to specific membrane curvatures. This article aims to decipher the interplay between the ultra-structure of septins at a molecular level and the membrane remodeling occurring at a micron scale. To this end, budding yeast, and mammalian septin complexes were recombinantly expressed and purified. A combination of in vitro assays was then used to analyze the self-assembly of septins at the membrane. Supported lipid bilayers (SLBs), giant unilamellar vesicles (GUVs), large unilamellar vesicles (LUVs), and wavy substrates were used to study the interplay between septin self-assembly, membrane reshaping, and membrane curvature.

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Septins are cytoskeletal proteins. They interact with lipid membranes and can sense but also generate membrane curvature at the micron scale. We describe in this protocol bottom-up in vitro methodologies for analyzing membrane deformations, curvature-sensitive septin binding, and septin filament ultrastructure.

その場で液体の SEM を用いた水中のベーマイト粒子のキャラクタリゼーション

要約

さらに動画を探す

この動画の章

研究室での惑星インテリア分化過程のシミュレーション

エクスブソリュート鉱物相の原子プローブ断層撮影解析

米国陸軍研究所で一括ナノ多結晶金属の処理

界面遷移帯における凝集表面形態の測定(ITZ)

クライオ集束イオンビームフライス加工と走査型電子顕微鏡・分光法の結合による液体-固体界面のナノスケール特性評価

Ex Vivoモデルにおける組織石灰化の包括的な特性評価

Ex Vivoモデルにおける組織石灰化の包括的な特性評価

Ex Vivoモデルにおける組織石灰化の包括的な特性評価

マリンTubewormでライブ光学および電子顕微鏡技術を用いた石灰化イベントのキャラクタリゼーション

セプチンの超微細構造組織、膜再形成、および曲率感度挙動をアッセイするためのボトムアップインビトロ法

その場で液体の SEM を用いた水中のベーマイト粒子のキャラクタリゼーション

要約

さらに動画を探す

この動画の章

研究室での惑星インテリア分化過程のシミュレーション

エクスブソリュート鉱物相の原子プローブ断層撮影解析

米国陸軍研究所で一括ナノ多結晶金属の処理

界面遷移帯における凝集表面形態の測定(ITZ)

クライオ集束イオンビームフライス加工と走査型電子顕微鏡・分光法の結合による液体-固体界面のナノスケール特性評価

Ex Vivoモデルにおける組織石灰化の包括的な特性評価

Ex Vivoモデルにおける組織石灰化の包括的な特性評価

Ex Vivoモデルにおける組織石灰化の包括的な特性評価

マリンTubewormでライブ光学および電子顕微鏡技術を用いた石灰化イベントのキャラクタリゼーション

セプチンの超微細構造組織、膜再形成、および曲率感度挙動をアッセイするためのボトムアップ インビトロ 法

セプチンの超微細構造組織、膜再形成、および曲率感度挙動をアッセイするためのボトムアップインビトロ法