Name: the rodent psychomotor vigilance test (rpvt): a method for assessing neurobehavioral performance in rats and mice
Uploaded: 2016-12-29T12:00:00.000+00:00
Duration: 7 min 47 s
Description: Watch this Scientific Journal Video about The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice at JoVE.com

This research was supported by NASA (NNX15AC17G to CMD), and by NASA cooperative agreement NCC 9-58 (E000010 to CMD, NBPF02802 and NBPF04201 to RDH) with the National Space Biomedical Research Institute.

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The authors have nothing to disclose.

本明細書に記載された方法論は、人間の文献で報告され、典型的なPVTのパフォーマンスに多くの面で遜色がラットでrPVT公演になります。現在の技術を使用して、一方が迅速rPVTを実行するために、ラットを訓練することができ、これらの技術を用いて得られた性能が明らか差別（応答正しいすなわち 、高レベルの両方の応答を時期尚早と経過の低レベル）により特徴付けられます。 Bei...

人間の精神運動警戒試験（PVT）は、ヒトに警戒し、持続的注意を測定するために広く使用されている、よく検証ツールであり、もともとデンジーズらによって開発されました。全体としてセッション内および個々のセッション内の時間間の両方、反応時間と注意（注意における早期の応答および失効の観点から例えば 、エラー）での安定性を評価するための1-3。長年にわたり、人間のPVTは修正され、さまざまな側面の注目の経時変化を追跡するために、4月11日に更新し、睡眠不足や疲労の変化に敏感であることが実証されており、薬物使用と被験者12の年齢によって影響されています、13。 10秒 - PVTは、刺激（典型的には、LEDナンバーディスプレイ）は2の後、典型的には、時間的にランダムに表示されたときに被写体が簡単に画面をタッチする一見単純な手順です。人間のバージョンでは、番号表示はミリ秒でインクリメントしたときトン停止します彼の画面は、このように被験者の反応時間（RT）を示す、タッチされました。警戒反応時間、2）人間の文献で「漏れの誤差」と呼ば経過の増大（及び通常は長さ&gt; 500ミリ秒）であるのRTのように定義され、3）増大は、1）減速示される減少します早期の応答（と呼ばれる「手数料のエラー」またはヒト文学における「偽開始」）。他の手段もまた、性別や年齢の違いなどの変数を検査するためのPVTを得ることができます。これらの対策の見直しのため、Basnerとデンジーズ4を参照してください。最後に、PVTは人間のリスク評価の一般的な領域で採用されており、成功したようなNASAのエクストリーム環境のミッションとして、軍事、航空、鉄道産業、第一応答者、および極限環境を含む業務領域の広い範囲の下で使用されていますオペレーション（NEEMO）、国際Mars500プロジェクト14、および国際SPACについてEステーション（ISS）。 ISSには、PVTは「反応セルフテスト」と呼ばれ、個別疲労関連のフィードバックとの宇宙飛行士を提供するために使用されている（ 例えば 、RTのか注目で経過で変化します）。 （やや似ています）単純反応時間のタスクの齧歯類のバージョンを持っているように人間のPVTは、何十年も使用されています。これは、ごく最近、しかしながら、人間のPVTに直接げっ歯類対応物は、文献に報告されているされていること。クリスティらは、ラットのための人間のPVTのバージョンを記載し、睡眠不足15,16以下の警戒の減少を報告しました。追加の最近の研究では、rPVT 17-19のバージョンを報告しています。これらのレポートは、さまざまな睡眠不足のテクニック以下の持続的注意の変化を記載しています。しかしながら、これらの研究からのデータはまた、例えば 、RESPOの総数のいくつかの場合において、40％以上（応答早期の高レベルを報告していますNSE）;このような性能は非常に人間との任意のPVTの公演とは違っています。人間の性能対げっ歯類でのこのような大きな違いは、PVTのげっ歯類バージョン対ヒトにおいて使用される特定のパラメータの違いによる可能性が高いです。例えば、クリスティら。 （ - 4秒foreperiod。1を使用しています人間のPVTの3分バージョンのBasner ら 5を参照してくださいが）10秒foreperiod -人間のPVTは通常2を採用しながら、7秒foreperiod -研究では、ランダムに変化3を採用しました。比較的短いforeperiod値の使用は、多くの場合、その応答「タイミング」動物をもたらすことができる、したがって、現在のげっ歯類rPVT研究で報告されているように偶発的補強を介して、早期応答の数を増加させ、促進することができます。 ここで説明rPVTのバージョンは、私たちの以前に発表された物品20をベースにしており、関連する技術および手順の詳細な説明を提供します。それは異なります以下の点でrPVTの以前に公開されたバージョンから：1）ラットを3の可変foreperiod値で訓練した - 10秒、および2）ラットは短い応答ウィンドウ内でのみ応答するので、迅速に対応しなければならなかった（また、「限定と呼ばれますrPVTの以前公開されたバージョンでは3.0秒）;）」を開催し、次の刺激開始は、（本研究で1.5秒を強化しました。精度の大幅な向上と早期の応答のレベルの低下によって示されるように、誤ったためにこれらの変更だけでなく、短いタイムアウトを使用して、刺激制御のより高いレベルをもたらしたで応答し。本報告書はまた、パフォーマンス変数で予測可能な変更点について説明します（ 例えば 、注意、のRTでの経過）を調べる警戒が21をデクリメントし、ときに人間に見られるものを平行ヒト」タスク上の時間」効果と対応-などの他の性能尺度を調べるとき刺激間隔（RSI）は、ヒトPVTで観察される効果<s&gt; 22アップ。 ここで説明rPVTの最終バージョンは、家の光をオンにすることによって開始する（ 図1を参照）。 3の可変間隔（foreperiod）の後 - 10秒経過すると、鼻を突くキーを1.5秒の最大のために照射されます。 （foreperiods期間の平等な分配を保証するために、値がランダムに200ミリ秒の単位に基づいて、3〜10秒の範囲で36可能な値のリストから交換せずに生成されます。）nosepokeキーのイルミネーションに動物のための信号であり、対応し、光発症後150〜1500ミリ秒の間に発生する応答は、45 mgのペレットで補強されています。補強応答の後、鼻を突くキーライト、家の光の両方がオフになっていると1秒の試行間の間隔は（ITIは、家の光オフ）が行なわれます。光発症前で鼻ポークは、家の光を消火によって通知された実験不測の事態から8秒タイムアウト（TO）を産生します。何の応答は1.5秒の解像度内で発生しない場合ponseウィンドウは、nosepoke光と家の光の両方がオフし、ITIが続いて起こる1秒されています。その後の裁判のための次のスケジュールforeperiod値は、前の試験中に発生した方、ITI 1秒または8秒のいずれかの後に開始されます。セッションは、通常、約200件の試験で構成され、30分後に終了（5日/週）、毎日行われています。これは平均して、約7.5秒の持続時間を有する各試験での結果。 最初は決勝まで逐次近似を強化することにより、チャンバ内の食品トレーのうち、鼻突くキーに対応するため2）手整形ラットを食物ペレットを取るために、ラットを適応）rPVT上の安定したベースライン性能は1によって達成されるシェーピング鼻が応答を突く、および3）rPVT手順（ すなわち 、foreperiod、ITI、TO、およびキー照明回）のパラメータは、徐々に各ラットは、各セッション中に実行しているどれだけうまくに応じて、セッションを介して調整されている毎日のセッションを行います（以下に詳細に記載）。

<table><tbody><tr><td>Modular rat operant chamber with stainless steel grid floor</td><td>Med Associates</td><td>ENV-008</td><td>Med Associates Inc.&lt;br/&gt; PO Box 319&lt;br/&gt; St. Albans, Vermont 05478,&lt;br/&gt; USA&lt;br/&gt; Phone: (802) 527-2343</td></tr><tr><td>Sound attenuating chamber&amp;nbsp;</td><td>Med Associates</td><td>ENV-022MD</td><td>Houses rat operant chamber</td></tr><tr><td>Houselight for rat</td><td>Med Associates</td><td>ENV-215M</td><td></td></tr><tr><td>1&quot; diameter rat nose poke response key</td><td>Med Associates</td><td>ENV-119M-1</td><td></td></tr><tr><td>Pellet receptacle, trough type</td><td>Med Associates</td><td>ENV-200R2M</td><td></td></tr><tr><td>Modular pellet dispenser for rat, 45-mg</td><td>Med Associates</td><td>ENV-203M-45</td><td></td></tr><tr><td>PCI Operating package for up to 8 operant chambers</td><td>Med Associates</td><td>MED-SYST-8</td><td></td></tr><tr><td>SmartCtrl</td><td>Med Associates</td><td>DIG-716P1</td><td>This catalog number has 8 outputs and 4 inputs which is the minimum needed to run the rPVT; SmartCtrl can also be purchased with 16 outputs and 8 inputs for more flexibility</td></tr><tr><td>Med-PC IV software</td><td>Med Associates</td><td>SOF-735</td><td></td></tr><tr><td>PC computer with PCI card slot</td><td>Any manfacturer (&lt;em&gt;e.g.&lt;/em&gt;, Dell)</td><td></td><td>Use of the PCI operating package requires a computer with a PCI card slot. Systems that use PCIe are available. Contact Med Associates for details.</td></tr><tr><td>Dustless Precision pellets 45-mg rodent grain-based diet</td><td>Bio Serv</td><td>FO165</td><td>Bio-Serv&lt;br/&gt; One 8th Street, Suite 1&lt;br/&gt; Frenchtown, NJ 08825, USA&lt;br/&gt; Phone: (800)-996-9908;&amp;nbsp;&amp;nbsp; Standard chow pellets are commonly used. Different pellets (&lt;em&gt;e.g.&lt;/em&gt;, sucrose) can be acquired from Bio Serv.</td></tr></tbody></table>

the rodent psychomotor vigilance test (rpvt): a method for assessing neurobehavioral performance in rats and mice

人間の精神運動警戒試験（PVT）は、疲労や持続的注意の変化を測定するために広く使用されている手順です。本記事では、注目の同様の側面（ すなわち 、パフォーマンスの精度、モータの速度、応答時期尚早、と注意で失効）PVT-と呼ばれる「rPVT」 -つまり対策のげっ歯類のバージョンについて説明します。データは、実験用ラットで使用される場合rPVTの短期および長期の両方の有用性を示すが提示されています。ラットは簡単rPVTを学び、基本的な手順を実行するために学ぶことは訓練未満の2週間かかります。一度、rPVTにおけるラットの性能がセッション時間（すなわち 、人間の「タイム・オン・全体での類似性、注意で経過、反応時間、警戒デクリメントを含め、人間のPVTでこれらの同じ性能の尺度との類似度の高い示し取得タスク &quot;効果）、および人間のため記載応答刺激間隔（RSI）の効果。このようにRPVTは人間のPVT性能と非常によく似て持続的注意上の変数の広い範囲の効果を評価するための非常に貴重なツールとなることができ、したがって、神経行動機能障害のための新規治療法を開発するために有用であり得ます。

ベースラインのラットrPVT公演ここでは詳細なパラメータを使用すると、122雄ラットの86.7パーセントは、9.0の平均内の最終rPVTパラメータに達した（SD = 10.13; N = 122）4.1に記載されたコンピュータの自動化トレーニングセッション（ - 4.9 rPVTトレーニング、私たちの経験では、ラットのみの約2％）がrPVTを獲得しません。現在の...

Watch this Scientific Journal Video about The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice at JoVE.com

The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice

人間の精神運動警戒試験（PVT）のラットのバージョンは、このようなパフォーマンスの精度、モータの速度、応答時期尚早、と注意で失効としてのヒト警戒の側面を含め、人間PVTを用いて測定したものと同様の注意の側面を測定することが記載されています。

げっ歯類精神運動警戒試験（rPVT）：ラットとマウスの神経行動のパフォーマンスを評価するための方法

The human Psychomotor Vigilance Test (PVT) is a widely used procedure for measuring changes in fatigue and sustained attention. The present article ...

the-rodent-psychomotor-vigilance-test-rpvt-method-for-assessing

Universidad de Cartagena UDEC

Research

JoVE Journal

Behavior

The Rodent Psychomotor Vigilance Test (rPVT): A Method for Assessing Neurobehavioral Performance in Rats and Mice

12.0K ビュー.  The Johns Hopkins University School of Medicine. 人間の精神運動警戒試験（PVT）のラットのバージョンは、このようなパフォーマンスの精度、モータの速度、応答時期尚早、と注意で失効としてのヒト警戒の側面を含め、人間PVTを用いて測定したものと同様の注意の側面を測定することが記載されています。 

ビデオ: The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice

Assessment of Sensorimotor Function in Mouse Models of Parkinson's Disease

Sensitive and reliable behavioral outcome measures are essential to the evaluation of potential therapeutic treatments in preclinical trials for many neurodegenerative diseases. In Parkinson's disease, sensorimotor tests sensitive to varying degrees of nigrostriatal dysfunction are fundamental for testing the efficacy of potential therapeutics. Reliable and quite elegant sensorimotor measures exist for rats, however many of these tests measure sensorimotor asymmetry within the rat and are not entirely suitable for the newer genetic mouse models of PD. We have put together a battery of sensorimotor tests inspired by the sensitive tests in rats and adapted for mice. The test battery highlighted in this study is chosen for a) its sensitivity in a wide variety of mouse models of PD, b) its ease in implementing into a study, and c) its low expense. These tests have proven useful in characterizing novel genetic mouse models of PD as well as in testing potential disease-modifying therapies.

In Parkinson's disease and movement disorders in general, sensitive and reliable behavioral assays are essential for testing novel potential therapeutics. Here, we describe a manageable battery of sensorimotor tests for mice that are sensitive to varying degrees of injury to the nigrostriatal system and useful for preclinical studies.

パーキンソン病のマウスモデルにおける感覚運動機能の評価

Sensitive and  reliable behavioral outcome measures are essential to the evaluation of  potential therapeutic treatments in preclinical trials for many  ...

行動学

Automated, Quantitative Cognitive/Behavioral Screening of Mice: For Genetics, Pharmacology, Animal Cognition and Undergraduate Instruction

We describe a high-throughput, high-volume, fully automated, live-in 24/7 behavioral testing system for assessing the effects of genetic and pharmacological manipulations on basic mechanisms of cognition and learning in mice. A standard polypropylene mouse housing tub is connected through an acrylic tube to a standard commercial mouse test box. The test box has 3 hoppers, 2 of which are connected to pellet feeders. All are internally illuminable with an LED and monitored for head entries by infrared (IR) beams. Mice live in the environment, which eliminates handling during screening. They obtain their food during two or more daily feeding periods by performing in operant (instrumental) and Pavlovian (classical) protocols, for which we have written protocol-control software and quasi-real-time data analysis and graphing software. The data analysis and graphing routines are written in a MATLAB-based language created to simplify greatly the analysis of large time-stamped behavioral and physiological event records and to preserve a full data trail from raw data through all intermediate analyses to the published graphs and statistics within a single data structure. The data-analysis code harvests the data several times a day and subjects it to statistical and graphical analyses, which are automatically stored in the &#34;cloud&#34; and on in-lab computers. Thus, the progress of individual mice is visualized and quantified daily. The data-analysis code talks to the protocol-control code, permitting the automated advance from protocol to protocol of individual subjects. The behavioral protocols implemented are matching, autoshaping, timed hopper-switching, risk assessment in timed hopper-switching, impulsivity measurement, and the circadian anticipation of food availability. Open-source protocol-control and data-analysis code makes the addition of new protocols simple. Eight test environments fit in a 48 in x 24 in x 78 in&#160;cabinet; two such cabinets (16 environments) may be controlled by one computer.

Fully automated system for measuring physiologically meaningful properties of the mechanisms mediating spatial localization, temporal localization, duration, rate&#160;and probability estimation, risk assessment, impulsivity, and the accuracy and precision of memory, in order to assess the effects of genetic and pharmacological manipulations on foundational mechanisms of cognition in mice.

マウスの自動化、定量的認知/行動スクリーニング：遺伝学、薬理学、動物認知と学部命令について

We describe a high-throughput, high-volume, fully automated, live-in 24/7 behavioral testing system for assessing the effects of genetic and ...

A Fully Automated Rodent Conditioning Protocol for Sensorimotor Integration and Cognitive Control Experiments

Rodents have been traditionally used as a standard animal model in laboratory experiments involving a myriad of sensory, cognitive, and motor tasks. Higher cognitive functions that require precise control over sensorimotor responses such as decision-making and attentional modulation, however, are typically assessed in nonhuman primates. Despite the richness of primate behavior that allows multiple variants of these functions to be studied, the rodent model remains an attractive, cost-effective alternative to primate models. Furthermore, the ability to fully automate operant conditioning in rodents adds unique advantages over the labor intensive training of nonhuman primates while studying a broad range of these complex functions.
Here, we introduce a protocol for operantly conditioning rats on performing working memory tasks. During critical epochs of the task, the protocol ensures that the animal's overt movement is minimized by requiring the animal to 'fixate' until a Go cue is delivered, akin to nonhuman primate experimental design. A simple two alternative forced choice task is implemented to demonstrate the performance. We discuss the application of this paradigm to other tasks.

A fully automated protocol for rodent operant conditioning is proposed. The protocol relies on precise temporal control of behavioral events to investigate the extent to which this control influences neural activity underlying sensorimotor integration and cognitive control experiments.

感覚統合と認知制御実験のための完全に自動化されたげっ歯類エアコンプロトコル

Rodents have been traditionally used as a standard animal model in laboratory experiments involving a myriad of sensory, cognitive, and motor tasks. ...

Behavioral Assessment of the Aging Mouse Vestibular System

Age related decline in balance performance is associated with deteriorating muscle strength, motor coordination and vestibular function. While a number of studies show changes in balance phenotype with age in rodents, very few isolate the vestibular contribution to balance under either normal conditions or during senescence. We use two standard behavioral tests to characterize the balance performance of mice at defined age points over the lifespan: the rotarod test and the inclined balance beam test. Importantly though, a custom built rotator is also used to stimulate the vestibular system of mice (without inducing overt signs of motion sickness). These two tests have been used to show that changes in vestibular mediated-balance performance are present over the murine lifespan. Preliminary results show that both the rotarod test and the modified balance beam test can be used to identify changes in balance performance during aging as an alternative to more difficult and invasive techniques such as vestibulo-ocular (VOR) measurements.

Motor control and balance performance are known to deteriorate with age. This paper presents a number of standard noninvasive behavioral tests with the addition of a simple rotary stimulus to challenge the vestibular system and show changes in balance performance in a murine model of aging.

高齢マウス前庭系の行動評価

Age related decline in balance performance is associated with deteriorating muscle strength, motor coordination and vestibular function. While a number of ...

The Attentional Set Shifting Task: A Measure of Cognitive Flexibility in Mice

Cognitive impairment, particularly involving dysfunction of circuitry within the prefrontal cortex (PFC), represents a core feature of many neuropsychiatric and neurodevelopmental disorders, including depression, post-traumatic stress disorder, schizophrenia and autism spectrum disorder. Deficits in cognitive function also represent the most difficult symptom domain&#160;to successfully treat, as serotonin reuptake inhibitors and tricyclic antidepressants have only modest effects. Functional neuroimaging studies and postmortem analysis of human brain tissue implicate the PFC as being a primary region of dysregulation in patients with these disorders. However, preclinical behavioral assays used to assess these deficits in mouse models which can be readily manipulated genetically and could provide the basis for studies of new treatment avenues have been underutilized. Here we describe the adaptation of a behavioral assay, the attentional set shifting task (AST), to be performed in mice to assess prefrontal cortex mediated cognitive deficits. The neural circuits underlying behavior during the AST are highly conserved across humans, nonhuman primates and rodents, providing excellent face, construct and predictive validity.

The goal of this protocol is to perform a behavioral assay such as the attentional set shifting task (AST) to assess prefrontal cortex-mediated cognitive flexibility in mice.

注意のセットシフトタスク：マウスにおける認知の柔軟性の測定

Cognitive impairment, particularly involving dysfunction of circuitry within the prefrontal cortex (PFC), represents a core feature of many ...

Operant Procedures for Assessing Behavioral Flexibility in Rats

Executive functions consist of multiple high-level cognitive processes that drive rule generation and behavioral selection. An emergent property of these processes is the ability to adjust behavior in response to changes in one&#8217;s environment (i.e., behavioral flexibility). These processes are essential to normal human behavior, and may be disrupted in diverse neuropsychiatric conditions, including schizophrenia, alcoholism, depression, stroke, and Alzheimer&#8217;s disease. Understanding of the neurobiology of executive functions has been greatly advanced by the availability of animal tasks for assessing discrete components of behavioral flexibility, particularly strategy shifting and reversal learning. While several types of tasks have been developed, most are non-automated, labor intensive, and allow testing of only one animal at a time. The recent development of automated, operant-based tasks for assessing behavioral flexibility streamlines testing, standardizes stimulus presentation and data recording, and dramatically improves throughput. Here, we describe automated strategy shifting and reversal tasks, using operant chambers controlled by custom written software programs. Using these tasks, we have shown that the medial prefrontal cortex governs strategy shifting but not reversal learning in the rat, similar to the dissociation observed in humans. Moreover, animals with a neonatal hippocampal lesion, a neurodevelopmental model of schizophrenia, are selectively impaired on the strategy shifting task but not the reversal task. The strategy shifting task also allows the identification of separate types of performance errors, each of which is attributable to distinct neural substrates. The availability of these automated tasks, and the evidence supporting the dissociable contributions of separate prefrontal areas, makes them particularly well-suited assays for the investigation of basic neurobiological processes as well as drug discovery and screening in disease models.

The ability to assess executive functions such as behavioral flexibility in rats is useful for investigating the neurobiology of cognition in both intact animals and disease models. Here we describe automated tasks for assessing strategy shifting and reversal learning, which are particularly sensitive to disruptions in prefrontal cortical networks.

ラットの行動の柔軟性を評価するためのオペラント手順

Executive functions consist of multiple high-level cognitive processes that drive rule generation and behavioral selection. An emergent property of these ...

Neurodevelopmental Reflex Testing in Neonatal Rat Pups

Neurodevelopmental reflex testing is commonly used in clinical practice to assess the maturation of the nervous system. Neurodevelopmental reflexes are also referred to as primitive reflexes. They are sensitive and consistent with later outcomes. Abnormal reflexes are described as an absence, persistence, reappearance, or latency of reflexes, which are predictive indices of infants that are at high risk for neurodevelopmental disorders. Animal models of neurodevelopmental disabilities, such as cerebral palsy, often display aberrant developmental reflexes, as would be observed in human infants. The techniques described assess a variety of neurodevelopmental reflexes in neonatal rats. Neurodevelopmental reflex testing offers the investigator a testing method that is not otherwise available in such young animals. The methodology presented here aims to assist investigators in examining developmental milestones in neonatal rats as a method of detecting early-onset brain injury and/or determining the effectiveness of therapeutic interventions. The methodology presented here aims to provide a general guideline for investigators.

Behavioral testing is the gold standard for determining outcomes following brain injury, and can identify the presence of developmental disabilities in infants and children. Neurodevelopmental reflexes are an early indicator of these abnormalities. A host of easily accomplished developmental reflex tests in the neonatal rodent were developed and described here.

新生ラット仔における神経発達レフレックステスト

Neurodevelopmental reflex testing is commonly used in clinical practice to assess the maturation of the nervous system. Neurodevelopmental reflexes are ...

Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects and outcomes that a particular disease or treatment has on the animal&#39;s wellbeing and health. There are numerous behavioral tests that may be applied. We selected a test for general locomotion, the open field test (OFT); a test for muscular strength, the mesh test (MT); and a test for coordination, the rotarod test (RR). Testing can be accomplished on a weekly or monthly basis. As a test for general locomotion, the OFT works by objectively monitoring movement parameters while the mouse is in an open field apparatus. The field is generally a 2&#39; x 2&#39; box, and the movements are recorded through laser sensing or through video capture. The mouse is placed in the center of the open field and allowed to move freely for the test. The MT uses the latency for a mouse to fall off an inverted screen as a measure of muscular strength. A mouse is placed on a screen, which is inverted over a clear box, and is timed for their latency to fall. Three trials are performed, with the best of the three trials scored for that day. A score of 60 s is the maximum time a mouse is left inverted. Mice are given a 5-min rest period between mesh test trials. Lastly, an accelerated protocol on the RR assesses motor coordination and endurance. During a trial, a mouse walks on a rotating rod as it increases in speed from 4 rpm to 40 rpm over 5 min. The trial ends when the mouse touches the magnetized pressure sensor upon falling. Each mouse undergoes three trials, and the best trial is scored for that day. This combined behavioral data allows for the global assessment of mobility, coordination, strength, and movement of the test animals. At least two out of the three behavioral testing measures must show improvement for an animal to qualify as having overall improved motor function.

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects of a disease or treatment on an animal's wellbeing. In order to globally assess motor functioning, we selected tests for general locomotion, muscular strength, and coordination: the open field test, the mesh test, and the rotarod test, respectively.

行動のバッテリー テスト評価の一般的な運動、筋肉の強さと連携マウス

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects and outcomes that a particular disease or treatment has on the ...

Behavioral Assessments of Spontaneous Locomotion in a Murine MPTP-induced Parkinson's Disease Model

Parkinson&#8217;s disease (PD) is a common neurodegenerative disorder disease, causing the phenomenon of shaking, rigidity, slowness of movement and dementia. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) can lead to some Parkinson&#8217;s-like symptoms by destroying dopaminergic neurons in the substantia nigra of the brain. It has been thus used to establish PD models in various animal studies. Here, mice receive MPTP injections (20 mg/kg/day) for seven days and the behavioral tests are performed on the eighth day. This model is adapted efficiently in the study of PD. The behavioral tests here include the cylinder test and the open field test. The cylinder experiment is used to detect the animals&#8217; ability to lift their front paws when put into a different environment. As the PD model mice show arching&#8212;the mouse arches its back&#8212;the number of paw liftings decrease. This test is easy to execute. The open field test is used to detect the amount of time the mice spend on running, walking, and remaining immobile. We analyze animals&#8217; movements in open field using software and obtain data. Lastly, we use L-DOPA, one of the most commonly used PD drugs, as one example to show how to apply this model to the study of PD drugs. Our results indicate that MPTP neurotoxicity induces motor deficit which can be mitigated by L-DOPA.

We describe establishment of a murine model for Parkinson's disease using MPTP, and behavioral assessments using cylinder and open field tests to measure motor function. We then use L-DOPA as one example to show how to apply this model in the study of PD drugs.

マウス MPTP 誘発パーキンソン病モデルにおける自発運動の行動評価

Parkinson&#8217;s disease (PD) is a common neurodegenerative disorder disease, causing the phenomenon of shaking, rigidity, slowness of movement and ...

A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice

Pharmacological and toxicological studies in neurodegeneration require comprehensive behavioral analysis in mice because motor dysfunctions and dysfunctions in mood and cognition are common and often shared symptoms in neurodegenerative diseases. Shown here is a behavioral test battery for motor, mood, and cognition, which can be repeatedly tested in a longitudinal study. This battery assesses the overall behavioral phenotype in mice by examining each domain of behavior with at least two independent well-accepted tests (i.e., open-field test and rotarod test for motor function, social interaction test, elevated plus maze test, and forced swim test for emotional function, and Morris water maze test and novel object recognition test for cognitive function). Therefore, this sensitive and comprehensive test battery is a powerful tool for the study of behavioral alternation in neurodegeneration.

A comprehensive behavioral test battery of motor skills, mood&#8212;including social interaction, depression, and anxiety&#8212;and cognition is designed for the repeated assessment of neurodegeneration-related behavioral changes in mice.

げっ歯類精神運動警戒試験（rPVT）：ラットとマウスの神経行動のパフォーマンスを評価するための方法

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この動画の章

パーキンソン病のマウスモデルにおける感覚運動機能の評価

マウスの自動化、定量的認知/行動スクリーニング：遺伝学、薬理学、動物認知と学部命令について

感覚統合と認知制御実験のための完全に自動化されたげっ歯類エアコンプロトコル

高齢マウス前庭系の行動評価

注意のセットシフトタスク：マウスにおける認知の柔軟性の測定

ラットの行動の柔軟性を評価するためのオペラント手順

新生ラット仔における神経発達レフレックステスト

行動のバッテリーテスト評価の一般的な運動、筋肉の強さと連携マウス

マウス MPTP 誘発パーキンソン病モデルにおける自発運動の行動評価

運動能力、気分およびマウスにおける認知の繰り返しの評価のための行動テストバッテリー

げっ歯類精神運動警戒試験（rPVT）：ラットとマウスの神経行動のパフォーマンスを評価するための方法

要約

さらに動画を探す

この動画の章

パーキンソン病のマウスモデルにおける感覚運動機能の評価

マウスの自動化、定量的認知/行動スクリーニング：遺伝学、薬理学、動物認知と学部命令について

感覚統合と認知制御実験のための完全に自動化されたげっ歯類エアコンプロトコル

高齢マウス前庭系の行動評価

注意のセットシフトタスク：マウスにおける認知の柔軟性の測定

ラットの行動の柔軟性を評価するためのオペラント手順

新生ラット仔における神経発達レフレックステスト

行動のバッテリー テスト評価の一般的な運動、筋肉の強さと連携マウス

マウス MPTP 誘発パーキンソン病モデルにおける自発運動の行動評価

運動能力、気分およびマウスにおける認知の繰り返しの評価のための行動テスト バッテリー

行動のバッテリーテスト評価の一般的な運動、筋肉の強さと連携マウス

運動能力、気分およびマウスにおける認知の繰り返しの評価のための行動テストバッテリー