Miglioramento in gel riduttive solfo-glycomics β-eliminazione completa per O-linked e di spettrometria di massa

1:16

Gel Excision and Removal of Gel-derived Contaminants

3:14

In-gel O-glycan Release by Reductive β-Elimination and Desalting on Cation-exchange Chromatography

6:02

Borate Removal, C18 Clean-up and Base Preparation for Permethylation

8:03

Permethylation of Released Glycans C18 Clean-up and Mass Spectrometry Analysis

10:33

Results: Recovery and Mass Spectral Analysis of Permethylated O-linked Glycans

12:30

Conclusion