Digestion des protéines, Ultrafiltration et chromatographie d’Exclusion afin d’optimiser l’isolement des Exosomes de sérum et de Plasma sanguin humain

1:06

Preparation of Crude Sample (Either Plasma or Serum) by Pelleting Larger Vesicles and Cellular Debris

2:04

Digestion of Non-exosomal Blood Proteins and Removal of Small Proteins/Peptides by Centrifuge Ultrafiltration

4:21

Purification of Vesicles by Size Exclusion Chromatography (SEC)

5:45

Concentration of Purified Exosomes/Total Protein Quantification and Quantification/Sizing of Exosomes by Nanoparticle Tracking Analysis (NTA)

7:09

Results: Increased Vesicle Yield and Exosome-specific Markers After Purification

8:15

Conclusion