该研究得到了河北省卫生厅青年科技项目的支持。（第20201046号）。

该方案遵循河北医科大学第三医院伦理委员会的指导方针。已获得患者的知情同意，以纳入他们和作为本研究一部分生成的数据。参加这项研究的患者年龄在 18-60 岁之间。该研究共纳入8名患者，5名女性和3名男性。
1.术前准备
<ol><li>使用以下纳入和排除标准将患者纳入本研究。<ol><li>使用这些纳入标准:通过膝关节 MRI 明确诊断为后交叉韧带囊肿的患者;有明显膝关节疼痛、过伸展疼痛和其他可能由后交叉韧带囊肿引起的症状的患者或需要关节镜治疗的合并半月板损伤患者;由于手术对他们的生活质量有影响而对手术感兴趣的患者。</li>
		<li>使用以下排除标准: 患有其他严重疾病且无法耐受手术的患者;拒绝手术治疗的患者;经MRI诊断为后交叉韧带囊肿但无症状的患者;联合前交叉韧带断裂或后交叉韧带断裂的患者。</li>
	</ol></li>
	<li>对所有患者使用全身麻醉或椎管内麻醉。将患者置于手术床上的仰卧位。将止血带套在大腿中部和上部的患肢上不超过 1 小时。用碘伏对患肢消毒 2 次，并铺设手术片。</li>
</ol>2. 关节镜入路的建立
<ol><li>使用驱血带将血液从患肢排出。从肢体末端到近端收紧驱血带。使每圈重叠 1/3。不要在每圈之间插入软组织。从手术肢体的软组织中排出血液。通过施加 50 kPa 的压力/力给止血带充气。</li>
	<li>建立前外侧通路，在膝外侧线上方 1 cm，髌腱缘外侧 1 cm。使用 0.5G 锋利刀片从皮肤到关节腔做一个约 11 厘米大小的切口。</li>
	<li>建立前内侧入路，在膝关节内侧关节线上方 1 cm，髌腱缘内侧 1 cm。使用 0.5G 锋利刀片从皮肤到关节腔做一个约 11 厘米大小的切口。</li>
	<li>将平行于胫骨平台的关节镜分别插入内侧和外侧进路朝向髁间窝。探查髌上关节囊、髌股关节、内侧转子清除、外侧转子沟、前交叉韧带、后交叉韧带、内侧半月板和外侧半月板。</li>
	<li>部分切除受伤的半月板或缝合受伤的半月板。拆下无接头主体。治疗关节内的所有损伤。</li>
	<li>建立正常的后内侧入路，如下所述。<ol><li>将膝盖弯曲 90°。将关节镜检查插入前内侧入路，以监测髁间窝，并将切换杆置于前外侧入路。</li>
		<li>在关节镜监测下，将后交叉韧带 （PCL） 和股内侧髁之间的交换棒插入后内侧关节囊。通常会有明显的滑入和滑出的感觉。</li>
		<li>将晶状体旋转到内侧后关节囊。见关节囊反向折叠形成的三角形区域、股内侧髁突和内侧半月板。</li>
		<li>关掉手术室的灯。观察膝关节后内侧皮肤表面的半透明区域（图2）。</li>
		<li>使用腰椎针辅助定位（图 3）。在半透明区域（通常位于股骨后髁后方和关节线近端）偏心穿刺到关节囊中。</li>
		<li>使用11G锋利刀片在皮肤上做一个约0.5厘米的小切口。在关节镜监测下将直夹插入关节囊。建立正常的后内侧入路。</li>
	</ol></li>
	<li>建立高后内侧入路，如下所述。<ol><li>将关节镜检查插入后内侧入路。将腰椎针插入关节方向，位于后内侧入路近端 2-3 厘米处。</li>
		<li>使腰椎针穿入内侧后髁、内侧半月板和后关节囊的三角形空间中的关节囊。</li>
		<li>在皮肤上做一个小切口。在关节镜监测下将直夹插入关节囊。建立高位后内侧入路。</li>
	</ol></li>
</ol>3.暴露和切除后交叉韧带囊肿
<ol><li>将关节镜检查插入高位后内侧入路。以正常的后内侧入路插入剃须刀（图 4）。</li>
	<li>使用剃须刀去除后交叉韧带和关节囊之间的滑膜组织。</li>
	<li>找到囊肿，它是后交叉韧带周围的半透明，凸起的滑膜（图1C）。切除囊肿壁，看到黄色和半透明的液体从囊肿壁流出，看到囊肿中的隔室状组织。</li>
	<li>完全切除后囊肿壁。避免损伤后交叉韧带和后血管和神经，并探索后交叉韧带而不受伤。</li>
	<li>在前外侧入路插入关节镜检查。在后交叉韧带的股骨末端看到囊肿的一部分。</li>
	<li>切除前囊肿壁，看到黄色和半透明的液体从囊肿壁流出。完全切除囊肿壁。</li>
	<li>切除前交叉韧带和后交叉韧带之间的 V 形空间中的滑膜组织。探查后交叉韧带并检查后交叉韧带是否损伤。</li>
	<li>将关节镜检查插入髌内侧入路。检查前交叉韧带和后交叉韧带之间的 V 形空间是否有残留的囊肿。</li>
	<li>在后内侧入路插入关节镜检查。检查有无残留囊肿。</li>
</ol>4. 切口闭合
<ol><li>将关节镜鞘插入前外侧入路。挤压髌上关节囊，完全排出关节内液。检查 0° 到 120° 的膝关节伸展和屈曲活动。</li>
	<li>用 4 号丝线缝合切口。</li>
</ol>5.术后康复
<ol><li>股四头肌锻炼:要求患者以仰卧位进行此锻炼。伸直患肢，勾起脚趾，慢慢将腿向上抬起至脚后跟约 15 厘米的高度，停留 3 秒钟，然后慢慢放下。</li>
	<li>关节释放训练:要求患者在坐在床边时进行此操作，并自行伸展和弯曲膝关节。</li>
	<li>要求患者在手术后第二天下床并适当活动，并逐渐恢复正常活动。</li>
	<li>手术后每 3 天更换一次敷料，手术后 14 天拆线。</li>
	<li>要求患者在手术后第一个月进行非对抗性活动，例如慢跑，并在手术后第三个月恢复正常活动。</li>
</ol>6. 术后护理和随访
<ol><li>要求患者在手术后 6 小时内不带枕头平躺。病人住院了大约3天。</li>
	<li>术后3个月，检查患者活动度是否恢复正常，术后12个月，进行患膝MRI检查，结合关节镜检查，检查患膝是否有不适，囊肿是否复发。</li>
</ol>

关节镜双后内侧入路用于 PCL 囊肿切除术

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作者声明，本研究不存在利益冲突。

后交叉韧带囊肿是一种罕见的疾病。膝关节韧带囊肿通常是在 MRI 或膝关节镜检查中发现的。膝韧带囊肿形成的原因是多种多样的，包括创伤后形成、胚胎发生过程中滑膜组织疝的形成以及间充质干细胞的增殖和形成。最近，创伤和组织刺激已被大多数专家认可 2,7,14。
大多数后交叉韧带囊肿患者无症状，?...

关节囊肿是囊性病变，囊肿液是一种透明的果冻状液体，可见于韧带、半月板、滑膜和膝关节的其他部位1,2。高机械应力很容易导致囊肿的形成，这就是为什么囊肿在膝关节中最常见 3,4，而贝克氏囊肿是最常见的囊肿类型5。膝关节交叉韧带囊肿很少见，在使用膝关节磁共振成像 （MRI） 扫描的病例中，有 0.2%-1.3% 偶然发生，在使用膝关节镜检查检查的患者中，有 0.6% 发生 6,7。后交叉韧带囊肿较为罕见，Brown 和 Dandy 报告说，在对 6,500 名患者进行膝关节镜检查后，他们只发现了 35 个韧带囊肿，只有 6 个来自后交叉韧带5。膝关节韧带囊肿不分性别或年龄均可发生，但在 20 至 40 岁的男性中更常见 4,8。
交叉韧带囊肿的病因尚不清楚。对于膝韧带囊肿的无创诊断，MRI可以清楚地显示囊肿的大小和位置之间的关系，是最准确的诊断方法4,6,9,10。关节镜下切除囊肿是最有效和推荐的治疗方法4,6,9,10。关节镜检查可以直接看到囊肿部位，完全去除囊肿壁，复发率极低，患者手术后恢复迅速7,9,10。后交叉韧带囊肿多呈分叶状或多房性，其中大部分主要位于后交叉韧带后面，在12.5%的患者中，囊肿主要位于后交叉韧带前方11（图1A，B）。
许多治疗方法可用于治疗后交叉韧带囊肿，例如超声和计算机断层扫描引导下的关节穿刺术。然而，几项研究表明，复发的风险可能更高，因此这些手术不会去除囊肿壁12。关节镜下切除囊肿是治疗后交叉韧带囊肿的金标准。关节镜手术可以完全切除囊肿壁，但仅通过前入路很难切除背交叉韧带囊肿。Abreu等人12 介绍了一种使用经鼻中隔门静脉关节镜切除PCL囊肿的方法，该方法安全有效。Tsai等13 报道，经鼻中隔入路切除后交叉韧带囊肿术在15例患者中成功，无复发。经鼻中隔入路需要增加后外侧入路，因此增加了损伤腓总神经和腘神经血管束的风险。为了避免这些风险，我们提出了一种手术方法，通过关节镜双后内侧入路治疗后交叉韧带囊肿。我们的方法不需要额外的后外侧方法;没有损伤腓总神经的风险。我们使用双后内侧入路，将整个手术过程保持在后间隔的内侧，从而减少损伤腘神经血管束的可能性。该技术产生相同的结果，同时比经鼻中隔方法更安全。这种手术方法特别适用于切除位于后交叉韧带背侧的囊肿。如果囊肿分隔或靠近胫骨末端，则手术方法更有利。

<table><tbody><tr><td>Arthroscopic sheath&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>72200829</td><td>6mm</td></tr><tr><td>Arthroscopy</td><td>smith&amp;amp;nephew</td><td>72202087</td><td>30 mm x 4 mm</td></tr><tr><td>Beam guide&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>72204925</td><td>5 mm x 3.6 m</td></tr><tr><td>Beam guide-arthroscopy end connector&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>2143</td><td></td></tr><tr><td>Beam guide-panel connector&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>2147</td><td></td></tr><tr><td>Blood-repellent belt</td><td>selani</td><td>tpe15100</td><td>15 cm x 1 m</td></tr><tr><td>Blunt puncture cone&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>4356</td><td>4 mm</td></tr><tr><td>Camera&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>72200561</td><td>NTSC/PAL</td></tr><tr><td>Coupler&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>72200315</td><td></td></tr><tr><td>DYONICS POWER II</td><td>smith&amp;amp;nephew</td><td>72200873</td><td>100-24VAC, 50/60Hz</td></tr><tr><td>DYONICS POWERMAX ELITE</td><td>smith&amp;amp;nephew</td><td>72200616</td><td></td></tr><tr><td>Endoscopic camera system</td><td>smith&amp;amp;nephew</td><td>72201919</td><td>560P NTSC/PAL</td></tr><tr><td>HD monitor&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>&amp;nbsp;LB500031</td><td>27 inch&amp;nbsp;</td></tr><tr><td>Hook probe&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>3312</td><td></td></tr><tr><td>Incisor plus platinum shaver&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>72202531</td><td>&amp;nbsp;4.5 mm</td></tr><tr><td>Lumbar needle&amp;nbsp; AN-E/S&amp;nbsp;&lt;img alt=&quot;Equation 1&quot; src=&quot;/files/ftp_upload/65620/65620eq01.jpg&quot; style=&quot;margin: auto;&quot;/&gt;</td><td>tuoren</td><td>AN-E/S&amp;nbsp;&lt;img alt=&quot;Equation 1&quot; src=&quot;/files/ftp_upload/65620/65620eq01.jpg&quot; style=&quot;margin: auto;&quot;/&gt;</td><td>1.6 mm x 80 mm</td></tr><tr><td>Micropunch,teardrop,left&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>7207602</td><td></td></tr><tr><td>Micropunch,teardrop,right&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>7207601</td><td></td></tr><tr><td>Micropunch,teardrop,straight&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>7207600</td><td></td></tr><tr><td>Pitbull Jr. Grasper&amp;nbsp;&amp;nbsp;</td><td>smith&amp;amp;nephew</td><td>14845</td><td></td></tr></tbody></table>

arthroscopic excision of posterior cruciate ligament cysts using a double posteromedial approach

膝关节交叉韧带囊肿是一种罕见的疾病。膝关节后交叉韧带囊肿比前交叉韧带囊肿少见。对于无症状的孤立性膝关节交叉韧带囊肿患者，建议保守治疗。有症状的膝关节交叉韧带囊肿多表现为膝关节过度屈曲痛、伸直疼痛、长时间站立或行走后出现膝关节不适等，严重影响生活质量，可进行手术治疗。手术治疗可分为超声引导下囊肿穿刺和液体提取手术和关节镜膀胱切除术。囊肿大多呈多层囊肿壁分叶状，囊肿液提取术并不能完全去除囊肿壁，而只是提取囊肿液，导致复发率高。关节镜手术可以完全切除囊肿壁，创伤小，复发率低，术后恢复快，因此关节镜切除术是最常见和首选的治疗方法。由于后交叉韧带囊肿多发生在韧带后方，我们通过在膝关节上增加双后内侧通路来切除囊肿壁，在直视下切除囊肿壁，操作简单，囊肿壁完全清除，创伤小，术后恢复快，无复发。在这里，切除了 8 个后交叉韧带囊肿，术后症状完全缓解，无手术并发症，1 年随访时无复发。

所有八名患者均成功手术，无任何并发症。8例患者中有7例为孤立性后交叉韧带囊肿，1例患者为内侧半月板损伤。所有患者术前的主要症状是膝关节过度屈曲疼痛、无法自由下蹲、长时间站立或长时间行走后膝后部疼痛、不适等。手术后，所有症状均得到缓解并消失（表1）。
7例单纯性后交叉韧带囊肿患者中，术后1年未发现特别不适。术后膝关节活动度较术前明...

Read this Scientific Article Protocol about Author Spotlight: Double Posteromedial Approach for Treating Posterior Cruciate Ligament Cysts at JoVE.com

作者聚焦: 双后内侧入路治疗后交叉韧带囊肿

我们提出了一种通过关节镜双后内侧入路治疗后交叉韧带囊肿的手术方法。

关节镜下使用双后内侧入路切除后交叉韧带囊肿

Cruciate ligament cysts of the knee are a rare condition. Posterior cruciate ligament cysts of the knee are less common than anterior cruciate ligament ...

膝关节交叉韧带囊肿，尤其是后部囊肿，并不常见。关节镜手术是首选的治疗方法，因为创伤最小、复发率低且恢复快。使其成为完全切除囊肿的最常见选择。在关节镜手术中，位于韧带后面的后交叉韧带囊肿采用双后内侧入路治疗。这种方法首先开发了用于完全切除囊肿的视觉方法;简化手术，创伤最小，恢复较少，不会复发。我们的手术方法特别适用于切除位于后交叉韧带背侧的囊肿。如果集合是分隔的或靠近胫骨末端，则第三种方法更有利。

arthroscopic-excision-posterior-cruciate-ligament-cysts-using-double

Universidad de Cartagena UDEC

Research

JoVE Journal

Medicine

Arthroscopic Excision of Posterior Cruciate Ligament Cysts Using a Double Posteromedial Approach

477 次观看.  The Third Hospital of Hebei Medical University. 膝关节交叉韧带囊肿，尤其是后部囊肿，并不常见。关节镜手术是首选的治疗方法，因为创伤最小、复发率低且恢复快。使其成为完全切除囊肿的最常见选择。在关节镜手术中，位于韧带后面的后交叉韧带囊肿采用双后内侧入路治疗。这种方法首先开发了用于完全切除囊肿的视觉方法;简化手术，创伤最小，恢复较少，不会复发。我们的手术方法特别适用于切除位于后交...

视频: 作者聚焦: 双后内侧入路治疗后交叉韧带囊肿

A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe

Enucleation is the process of retrieving the ocular globe from a cadaveric donor leaving the rest of the globe undisturbed. Excision refers to the retrieval of ocular tissues, especially cornea, by cutting it separate from the ocular globe. Evisceration is the process of removing the internal organs referred here as retina. The ocular globe consists of the cornea, the sclera, the vitreous body, the lens, the iris, the retina, the choroid, muscles etc (Suppl. Figure 1). When a patient is suffering from corneal damage, the cornea needs to be removed and a healthy one must be transplanted by keratoplastic surgeries. Genetic disorders or defects in retinal function can compromise vision. Human ocular globes can be used for various surgical procedures such as eye banking, transplantation of human cornea or sclera and research on ocular tissues. However, there is little information available on human corneal and retinal excision, probably due to the limited accessibility to human tissues. Most of the studies describing similar procedures are performed on animal models. Research scientists rely on the availability of properly dissected and well-conserved ocular tissues in order to extend the knowledge on human eye development, homeostasis and function. As we receive high amount of ocular globes out of which approximately 40% (Table 1) of them are used for research purposes, we are able to perform huge amount of experiments on these tissues, defining techniques to excise and preserve them regularly.
The cornea is an avascular tissue which enables the transmission of light onto the retina and for this purpose should always maintain a good degree of transparency. Within the cornea, the limbus region, which is a reservoir of the stem cells, helps the reconstruction of epithelial cells and restricts the overgrowth of the conjunctiva maintaining corneal transparency and clarity. The size and thickness of the cornea are critical for clear vision, as changes in either of them could lead to distracted, unclear vision. The cornea comprises of 5 layers; a) epithelium, b) Bowman's layer, c) stroma, d) Descemet's membrane and e) endothelium. All layers should function properly to ensure clear vision4,5,6. The choroid is the intermediate tunic between the sclera and retina, bounded on the interior by the Bruch's membrane and is responsible for blood flow in the eye. The choroid also helps to regulate the temperature and supplies nourishment to the outer layers of the retina5,6. The retina is a layer of nervous tissue that covers the back of the ocular globe (Suppl. Figure 1) and consists of two parts: a photoreceptive part and a non-receptive part. The retina helps to receive the light from the cornea and lens and converts it into the chemical energy eventually transmitted to the brain with help of the optic nerve5,6.
The aim of this paper is to provide a protocol for the dissection of corneal and retinal tissues from human ocular globes. Avoiding cross-contamination with adjacent tissues and preserving RNA integrity is of fundamental importance as such tissues are indispensable for research purposes aimed at (i) characterizing the transcriptome of the ocular tissues, (ii) isolating stem cells for regenerative medicine projects, and (iii) evaluating histological differences between tissues from normal/affected subjects. In this paper we describe the technique we currently use to remove the cornea, the choroid and retinal tissues from an ocular globe. Here we provide a detailed protocol for the dissection of the human ocular globe and the excision of corneal and retinal tissues. The accompanying video will help researchers to learn an appropriate technique for the retrieval of precious human tissues which are difficult to find regularly.

A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe

The paper describes a simplified technique to excise corneal and to eviscerate retinal tissues from the ocular globe of human cadaveric donors. The technique described here will help to excise good quality tissues to be used for transplantation, surgical or research purposes without damaging other tissues of the ocular globe.

简化技术<em&gt;原位</em&gt;切除角膜和视网膜组织剜人眼地球

Enucleation is the process of retrieving the ocular globe from a cadaveric donor leaving the rest of the globe undisturbed. Excision refers to the ...

科研

医学

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

Injury to the ACL is a commonly encountered problem in active individuals. Even partial tears of this intra-articular knee ligament lead to biomechanical deficiencies that impair function and stability. Current options for the treatment of partial ACL tears range from nonoperative, conservative management to multiple surgical options, such as: thermal modification, single-bundle repair, complete reconstruction, and reconstruction of the damaged portion of the native ligament. Few studies, if any, have demonstrated any single method for management to be consistently superior, and in many cases patients continue to demonstrate persistent instability and other comorbidities.
The goal of this study is to identify a potential cell source for utilization in the development of a tissue engineered patch that could be implemented in the repair of a partially torn ACL. A novel protocol was developed for the expansion of cells derived from patients undergoing ACL reconstruction. To isolate the cells, minced hACL tissue obtained during ACL reconstruction was digested in a Collagenase solution. Expansion was performed using DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). The cells were then stored at -80 &#186;C or in liquid nitrogen in a freezing medium consisting of DMSO, FBS and the expansion medium. After thawing, the hACL derived cells were then seeded onto a tissue engineered scaffold, PLAGA (Poly lactic-co-glycolic acid) and control Tissue culture polystyrene (TCPS). After 7 days, SEM was performed to compare cellular adhesion to the PLAGA versus the control TCPS. Cellular morphology was evaluated using immunofluorescence staining. SEM (Scanning Electron Microscope) micrographs demonstrated that cells grew and adhered on both PLAGA and TCPS surfaces and were confluent over the entire surfaces by day 7. Immunofluorescence staining showed normal, non-stressed morphological patterns on both surfaces. This technique is promising for applications in ACL regeneration and reconstruction.

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

For future applications as a patch to repair partial tears of the Anterior Cruciate Ligament (ACL), human ACL derived cells were isolated from tissue obtained during reconstructive procedures, expanded in vitro and grown on tissue engineered scaffolds. Cellular adhesion and morphology was then performed to confirm biocompatibility on scaffold surface.

手术取出，分离和<em&gt;在体外</em&gt;扩展人类前十字韧带衍生细胞组织工程的应用

Injury to the ACL is a commonly encountered problem in active individuals. Even partial tears of this intra-articular knee ligament lead to biomechanical ...

生物工程

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without intervention. Current treatment strategies include ligament reconstruction with either autograft or allograft, which each have their associated limitations. Thus, there is interest in designing a tissue-engineered graft for use in ACL reconstruction. We describe the fabrication of an electrospun polymer graft for use in ACL tissue engineering. This polycaprolactone graft is biocompatible, biodegradable, porous, and is comprised of aligned fibers. Because an animal model is necessary to evaluate such a graft, this paper describes an intra-articular athymic rat model of ACL reconstruction that can be used to evaluate engineered grafts, including those seeded with xenogeneic cells. Representative histology and biomechanical testing results at 16 weeks postoperatively are presented, with grafts tested immediately post-implantation and contralateral native ACLs serving as controls. The present study provides a reproducible animal model with which to evaluate tissue engineered ACL grafts, and demonstrates the potential of a regenerative medicine approach to treatment of ACL rupture.

Animal models are important tools for the evaluation of tissue-engineered grafts. This paper presents the protocol for preparing an electrospun biodegradable polymer graft for use in anterior cruciate ligament tissue engineering, as well as a surgical protocol for implantation in a rat model.

无胸​​腺大鼠模型工程前交叉韧带移植评估

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without ...

A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys

Polycystic kidney disease (PKD) is one of the most common causes of end-stage kidney disease, a devastating disease for which there is no cure. The molecular mechanisms leading to cyst formation in PKD remain somewhat unclear, but many genes are thought to be involved. Wnt5a is a non-canonical glycoprotein that regulates a wide range of developmental processes. Wnt5a works through the planar cell polarity (PCP) pathway that regulates oriented cell division during renal tubular cell elongation. Defects of the PCP pathway have been found to cause kidney cyst formation. Our paper describes a method for developing a zebrafish cystic kidney disease model by knockdown of the wnt5a gene with wnt5a antisense morpholino (MO) oligonucleotides. Tg(wt1b:GFP) transgenic zebrafish were used to visualize kidney structure and kidney cysts following wnt5a knockdown. Two distinct antisense MOs (AUG - and splice-site) were used and both resulted in curly tail down phenotype and cyst formation after wnt5a knockdown. Injection of mouse Wnt5a mRNA, resistant to the MOs due to a difference in primary base pair structure, rescued the abnormal phenotype, demonstrating that the phenotype was not due to &#8220;off-target&#8221; effects of the morpholino. This work supports the validity of using a zebrafish model to study wnt5a function in the kidney.

A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys

We describe a method of generating a possible zebrafish model of polycystic kidney disease. We used Tg(wt1b:GFP) fish to visualize kidney structure. Knockdown of wnt5a was by morpholino injection. Pronephric cyst formation after wnt5a knockdown was observed in this GFP transgenic zebrafish.

多囊肾一种可能的斑马鱼型号:击倒<em&gt; Wnt5a的</em&gt;原因在斑马鱼肾囊肿

Polycystic kidney disease (PKD) is one of the most common causes of end-stage kidney disease, a devastating disease for which there is no cure. The ...

Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease

Cardiovascular disease is a broad term describing disease of the heart and/or blood vessels. The main blood vessel supplying the body with oxygenated blood is the aorta. The aorta may become affected in diseases such as atherosclerosis and aneurysm. Researchers investigating these diseases would benefit from direct observation of the aorta to characterize disease progression as well as to evaluate efficacy of potential therapeutics. The goal of this protocol is to describe proper isolation and excision of the aorta to aid investigators researching cardiovascular disease. Isolation and excision of the aorta allows investigators to look at gross morphometric changes as wells as allowing them to preserve and stain the tissue to look at histologic changes if desired. The aorta may be used for molecular studies to evaluate protein and gene expression to discover targets of interest and mechanisms of action. This technique is superior to imaging modalities as they have inherent limitations in technology and cost. Additionally, primary isolated cells from a freshly isolated and excised aorta can allowing researchers to perform further in situ and in vitro assays. The isolation and excision of the aorta has the limitation of having to sacrifice the animal however, in this case the benefits outweigh the harm as it is the most versatile technique in the study of aortic disease.

Pathology of the aorta can lead to severe morbidity and mortality, therefore research of disease progression and potential therapies is warranted. Here, we present a protocol to isolate and excise the murine aorta to aid researchers in their investigation of cardiovascular disease.

隔离和小鼠主动脉切除;心血管疾病研究的一个多功能的技术

Cardiovascular disease is a broad term describing disease of the heart and/or blood vessels. The main blood vessel supplying the body with oxygenated ...

Localization, Identification, and Excision of Murine Adipose Depots

Obesity has increased dramatically in the last few decades and affects over one third of the adult US population. The economic effect of obesity in 2005 reached a staggering sum of $190.2 billion in direct medical costs alone. Obesity is a major risk factor for a wide host of diseases. Historically, little was known regarding adipose and its major and essential functions in the body. Brown and white adipose are the two main types of adipose but current literature has identified a new type of fat called brite or beige adipose. Research has shown that adipose depots have specific metabolic profiles and certain depots allow for a propensity for obesity and other related disorders. The goal of this protocol is to provide researchers the capacity to identify and excise adipose depots that will allow for the analysis of different factorial effects on adipose; as well as the beneficial or detrimental role adipose plays in disease and overall health. Isolation and excision of adipose depots allows investigators to look at gross morphological changes as well as histological changes. The adipose isolated can also be used for molecular studies to evaluate transcriptional and translational change or for in vitro experimentation to discover targets of interest and mechanisms of action. This technique is superior to other published techniques due to the design allowing for isolation of multiple depots with simplicity and minimal contamination.

Due to the drastic and negative connection between obesity and other comorbidities, research on the role adipose plays in disease and overall health is warranted. We present a protocol for the isolation and excision of adipose depots allowing for the study of adipose using in situ and in vitro methods.

本地化识别，切除脂肪小鼠油库，并

Obesity has increased dramatically in the last few decades and affects over one third of the adult US population. The economic effect of obesity in 2005 ...

Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus

In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several studies have focused their attention on mesenchymal stem cells derived from adipose tissue. Adipose-derived stem cells (ADSCs) exhibit morphological characteristics and properties similar to other mesenchymal cells, and are able to differentiate into several cellular lines. Moreover, these cells are also widely available in the subcutaneous tissue, representing 10 - 30% of the normal body weight, with a concentration of 5,000 cells per gram of tissue.
In the presented technique, the first step involves harvesting ADSCs from the abdomen and a process of microfracture and purification; next, the surgical procedure is performed entirely arthroscopically, with less soft tissue dissection, better joint visualization, and a faster recovery compared with standard open procedures. Arthroscopy is characterized by a first phase in which the lesion is identified, isolated, and prepared with microperforations; the second step, performed dry, involves injection of adipose tissue at the level of the lesion.
Between January 2016 and September 2016, four patients underwent arthroscopic treatment of osteochondral lesion of the talus with microfractured and purified adipose tissue. All patients reported clinical improvement six months after surgery with no reported complications. Functional scores at the latest follow-up are encouraging and confirm that the technique provides reliable pain relief and improvements in patients with osteochondral lesion of the talus.

The aim of this study is to report a protocol for the arthroscopic treatment of osteochondral lesions of the talus using microfractured and purified adipose-derived stem cells.

自体 Microfractured 与纯化脂肪组织在关节镜下治疗距骨软骨病变

In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several ...

An Effective Surgical Strategy for Anteromedial Osteoarthritis of the Knee with Simultaneous Popliteal Cysts Drainage

Unicompartmental knee arthroplasty (UKA) is an established treatment option for anteromedial osteoarthritis, and popliteal cysts are a common finding in the knee among patients with chronic osteoarthritis pain. The two are so closely related that popliteal cysts are commonly discovered during the unicompartmental knee arthroplasty preoperative examination. However, only a few reports exist on the management and outcome of popliteal cysts in the patients receiving UKA for knee osteoarthritis (OA) and popliteal cysts. As such, it is crucial to evaluate different treatment strategies and their management of popliteal cysts. In this paper, we evaluate a surgical strategy for patients with knee anteromedial osteoarthritis and symptomatic popliteal cysts. These patients were treated with UKA and internal drainage of the popliteal cyst. The results shown here, spanning 1-year post-operation follow-up, demonstrated that UKA and internal drainage is an effective surgical protocol for treating anteromedial osteoarthritis with symptomatic popliteal cysts.

<meta charset="utf8"></head><body>膝关节前内侧骨关节炎同时进行腘窝囊肿引流的有效手术策略

Popliteal cysts are frequently found during the pre-operative examination of patients with osteoarthritis being prepared for the unicompartmental knee arthroplasty. These symptomatic cysts usually require treatment. To do this, a unicompartmental knee arthroplasty was performed with internal drainage of the popliteal cyst under the same anesthetic.

膝关节前内侧骨关节炎同时进行腘窝囊肿引流的有效手术策略

Unicompartmental knee arthroplasty (UKA) is an established treatment option for anteromedial osteoarthritis, and popliteal cysts are a common finding in ...

Arthroscopic Repair of Medial Meniscus Posterior Root Tear with Adjustable Titanium Plate Fixation

Medial meniscus injury is one of the most prevalent knee disorders, with posterior root tears occurring in approximately 10% to 21% of affected individuals. A posterior root tear disrupts the meniscus's continuous annular structure, compromising its ability to absorb pressure and protect the knee's articular cartilage. If left untreated, this can result in increased stress on the cartilage, leading to conditions such as varus deformity and accelerated joint degeneration. Partial removal (resection) of the medial meniscus further exacerbates these issues, often leading to quicker joint deterioration. Repairing medial meniscus posterior root tears (MMPRTs) plays a crucial role in restoring the meniscus's structural integrity and improving knee biomechanics. Studies have shown that compared to partial medial meniscectomy or conservative treatments, repairing MMPRTs enhances clinical outcomes and significantly delays the onset of arthritis. This article details a surgical procedure that uses a single-suture, double-loop, adjustable titanium plate fixation technique under arthroscopy for effective MMPRT repair.

This article describes a surgical approach to repairing a medial meniscus posterior root tear (MMPRT) using a single-suture, double-loop, adjustable titanium plate internal fixation under arthroscopy.

关节镜下使用双后内侧入路切除后交叉韧带囊肿

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摘要

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简化技术

手术取出，分离和

无胸腺大鼠模型工程前交叉韧带移植评估

多囊肾一种可能的斑马鱼型号:击倒

隔离和小鼠主动脉切除;心血管疾病研究的一个多功能的技术

本地化识别，切除脂肪小鼠油库，并

自体 Microfractured 与纯化脂肪组织在关节镜下治疗距骨软骨病变

膝关节前内侧骨关节炎同时进行腘窝囊肿引流的有效手术策略

膝关节前内侧骨关节炎同时进行腘窝囊肿引流的有效手术策略

关节镜修复内侧半月板后根撕裂与可调节钛板固定

关节镜下使用双后内侧入路切除后交叉韧带囊肿

副本

摘要

探索更多视频

此视频中的章节

简化技术

手术取出，分离和

无胸​​腺大鼠模型工程前交叉韧带移植评估

多囊肾一种可能的斑马鱼型号:击倒

隔离和小鼠主动脉切除;心血管疾病研究的一个多功能的技术

本地化识别，切除脂肪小鼠油库，并

自体 Microfractured 与纯化脂肪组织在关节镜下治疗距骨软骨病变

膝关节前内侧骨关节炎同时进行腘窝囊肿引流的有效手术策略

膝关节前内侧骨关节炎同时进行腘窝囊肿引流的有效手术策略

关节镜修复内侧半月板后根撕裂与可调节钛板固定

无胸腺大鼠模型工程前交叉韧带移植评估